Quantitative analysis of histone demethylase probes using fluorescence polarization.

We previously reported methylstat as a selective inhibitor of jumonji C domain-containing histone demethylases (JHDMs). Herein, we describe the synthesis of a fluorescent analogue of methylstat and its application as a tracer in fluorescence polarization assays. Using this format, we have evaluated...

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Main Authors: Xu, W, Podoll, J, Dong, X, Tumber, A, Oppermann, U, Wang, X
格式: Journal article
语言:English
出版: 2013