Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro.
The effect of protocatechuic acid (PCA) from Alpinia oxyphylla and catapol from Rehmannia on the proliferation capacity of human adipose tissue-derived stromal cells (hADSCs) was investigated in vitro. Cell counts showed that treatment of hADSCs with PCA for 48 h increased the cell number in a dose-...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1797065101472169984 |
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| author | Wang, H Liu, T Zhu, Y Guan, S Ma, X Cui, Z |
| author_facet | Wang, H Liu, T Zhu, Y Guan, S Ma, X Cui, Z |
| author_sort | Wang, H |
| collection | OXFORD |
| description | The effect of protocatechuic acid (PCA) from Alpinia oxyphylla and catapol from Rehmannia on the proliferation capacity of human adipose tissue-derived stromal cells (hADSCs) was investigated in vitro. Cell counts showed that treatment of hADSCs with PCA for 48 h increased the cell number in a dose-dependent manner, while no obvious effect of catapol on the proliferation of hADSCs was observed. In addition, the cell number of hADSCs treated by 1.5 mM PCA increased in a time-dependent manner. The flow cytometric analysis of DNA content demonstrated the cell cycle progress from the G0/G1 phase to the S phase. Western blot analysis revealed the elevated expression of cyclin D1 in hADSCs induced by PCA treatment. Cyclin D1-siRNA transfection significantly inhibit the promotion of cell proliferation by PCA. Furthermore, the flow cytometric analysis of the cell surface antigens and the multidifferential potential tests of PCA-treated hADSCs showed that the cells retained their functional characteristics of multipotential mesenchymal progenitors. It is concluded that PCA can effectively up-regulate the proliferation of hADSCs. |
| first_indexed | 2024-03-06T21:23:50Z |
| format | Journal article |
| id | oxford-uuid:4269a591-f136-4a58-8d92-b5f3b30ab807 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:23:50Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:4269a591-f136-4a58-8d92-b5f3b30ab8072022-03-26T14:49:18ZEffect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4269a591-f136-4a58-8d92-b5f3b30ab807EnglishSymplectic Elements at Oxford2009Wang, HLiu, TZhu, YGuan, SMa, XCui, ZThe effect of protocatechuic acid (PCA) from Alpinia oxyphylla and catapol from Rehmannia on the proliferation capacity of human adipose tissue-derived stromal cells (hADSCs) was investigated in vitro. Cell counts showed that treatment of hADSCs with PCA for 48 h increased the cell number in a dose-dependent manner, while no obvious effect of catapol on the proliferation of hADSCs was observed. In addition, the cell number of hADSCs treated by 1.5 mM PCA increased in a time-dependent manner. The flow cytometric analysis of DNA content demonstrated the cell cycle progress from the G0/G1 phase to the S phase. Western blot analysis revealed the elevated expression of cyclin D1 in hADSCs induced by PCA treatment. Cyclin D1-siRNA transfection significantly inhibit the promotion of cell proliferation by PCA. Furthermore, the flow cytometric analysis of the cell surface antigens and the multidifferential potential tests of PCA-treated hADSCs showed that the cells retained their functional characteristics of multipotential mesenchymal progenitors. It is concluded that PCA can effectively up-regulate the proliferation of hADSCs. |
| spellingShingle | Wang, H Liu, T Zhu, Y Guan, S Ma, X Cui, Z Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro. |
| title | Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro. |
| title_full | Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro. |
| title_fullStr | Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro. |
| title_full_unstemmed | Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro. |
| title_short | Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro. |
| title_sort | effect of protocatechuic acid from alpinia oxyphylla on proliferation of human adipose tissue derived stromal cells in vitro |
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