Impairment of invasion and maturation and decreased selectivity of Plasmodium falciparum in G6PD Viangchan and Mahidol variants
<p><i><strong>Background.</strong></i> Protection against <i>Plasmodium falciparum</i> is observed in a population deficient in glucose-6-phosphate dehydrogenase (G6PD), particularly in African and Mediterranean regions. However, such protection remains unkn...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2021
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| Summary: | <p><i><strong>Background.</strong></i> Protection against <i>Plasmodium falciparum</i> is observed in a population deficient in glucose-6-phosphate dehydrogenase (G6PD), particularly in African and Mediterranean regions. However, such protection remains unknown among G6PD-deficient individuals in Southeast Asia.</p>
<p><i><strong>Methods.</strong></i> In this study, we assessed the invasion and maturation of <i>P falciparum</i> K1 in a culture of erythrocytes isolated from Thai subjects carrying Viangchan (871G > A) and Mahidol (487G > A).</p>
<p><i><strong>Results.</strong></i> We found that the parasites lost their ability to invade hemizygous and homozygous G6PD-deficient erythrocytes of Viangchan and Mahidol variants in the second and third cycles of intraerythrocytic development. It is interesting to note that <i>P falciparum</i> parasites selectively grew in erythrocytes from hemi- and homozygous genotypes with normal G6PD activity. Moreover, externalization of phosphatidylserine upon <i>P falciparum</i> infection was significantly increased only in Viangchan hemizygous variant cells.</p>
<p><i><strong>Conclusions.</strong></i> This study is the first to show that blockage of invasion in long-term culture and potentially enhanced removal of parasitized erythrocytes were observed for the first time in erythrocytes from Viangchan and Mahidol G6PD-deficient individuals.</p> |
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