Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot: Variability of the memory B cell ELISpot assay.

Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot: Variability of the memory B cell ELISpot assay.

The ELISpot assay is used in vaccine studies for the quantification of antigen-specific memory B cells (BMEM), and can be performed using cryopreserved samples. The effects of cryopreservation on BMEM detection and the consistency of cultured ELISpot assays when performed by different operators or l...

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Detalles Bibliográficos
Main Authors: Trück, J, Mitchell, R, Thompson, A, Morales-Aza, B, Clutterbuck, E, Kelly, D, Finn, A, Pollard, A
Formato: Journal article
Idioma:English
Publicado: 2014

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