Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot: Variability of the memory B cell ELISpot assay.
The ELISpot assay is used in vaccine studies for the quantification of antigen-specific memory B cells (BMEM), and can be performed using cryopreserved samples. The effects of cryopreservation on BMEM detection and the consistency of cultured ELISpot assays when performed by different operators or l...
Հիմնական հեղինակներ: | Trück, J, Mitchell, R, Thompson, A, Morales-Aza, B, Clutterbuck, E, Kelly, D, Finn, A, Pollard, A |
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Ձևաչափ: | Journal article |
Լեզու: | English |
Հրապարակվել է: |
2014
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Նմանատիպ նյութեր
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Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot.
: Trück, J, և այլն
Հրապարակվել է: (2014) -
Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children.
: Blanchard-Rohner, G, և այլն
Հրապարակվել է: (2010) -
Comparison of a limiting dilution assay and ELISpot for detection of memory B-cells before and after immunisation with a protein-polysaccharide conjugate vaccine in children
: Blanchard-Rohner, G, և այլն
Հրապարակվել է: (2010) -
Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays
: Ramu A. Subbramanian, և այլն
Հրապարակվել է: (2012-07-01) -
Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT
: Morse Michael A, և այլն
Հրապարակվել է: (2005-07-01)