The Listeria transcriptional landscape from saprophytism to virulence.
The bacterium Listeria monocytogenes is ubiquitous in the environment and can lead to severe food-borne infections. It has recently emerged as a multifaceted model in pathogenesis. However, how this bacterium switches from a saprophyte to a pathogen is largely unknown. Here, using tiling arrays and...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1826269524800831488 |
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| author | Toledo-Arana, A Dussurget, O Nikitas, G Sesto, N Guet-Revillet, H Balestrino, D Loh, E Gripenland, J Tiensuu, T Vaitkevicius, K Barthelemy, M Vergassola, M Nahori, M Soubigou, G Régnault, B Coppée, J Lecuit, M Johansson, J Cossart, P |
| author_facet | Toledo-Arana, A Dussurget, O Nikitas, G Sesto, N Guet-Revillet, H Balestrino, D Loh, E Gripenland, J Tiensuu, T Vaitkevicius, K Barthelemy, M Vergassola, M Nahori, M Soubigou, G Régnault, B Coppée, J Lecuit, M Johansson, J Cossart, P |
| author_sort | Toledo-Arana, A |
| collection | OXFORD |
| description | The bacterium Listeria monocytogenes is ubiquitous in the environment and can lead to severe food-borne infections. It has recently emerged as a multifaceted model in pathogenesis. However, how this bacterium switches from a saprophyte to a pathogen is largely unknown. Here, using tiling arrays and RNAs from wild-type and mutant bacteria grown in vitro, ex vivo and in vivo, we have analysed the transcription of its entire genome. We provide the complete Listeria operon map and have uncovered far more diverse types of RNAs than expected: in addition to 50 small RNAs (<500 nucleotides), at least two of which are involved in virulence in mice, we have identified antisense RNAs covering several open-reading frames and long overlapping 5' and 3' untranslated regions. We discovered that riboswitches can act as terminators for upstream genes. When Listeria reaches the host intestinal lumen, an extensive transcriptional reshaping occurs with a SigB-mediated activation of virulence genes. In contrast, in the blood, PrfA controls transcription of virulence genes. Remarkably, several non-coding RNAs absent in the non-pathogenic species Listeria innocua exhibit the same expression patterns as the virulence genes. Together, our data unravel successive and coordinated global transcriptional changes during infection and point to previously unknown regulatory mechanisms in bacteria. |
| first_indexed | 2024-03-06T21:26:25Z |
| format | Journal article |
| id | oxford-uuid:433c4b63-43a6-41bf-9799-4ae971dce1e0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:26:25Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:433c4b63-43a6-41bf-9799-4ae971dce1e02022-03-26T14:54:17ZThe Listeria transcriptional landscape from saprophytism to virulence.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:433c4b63-43a6-41bf-9799-4ae971dce1e0EnglishSymplectic Elements at Oxford2009Toledo-Arana, ADussurget, ONikitas, GSesto, NGuet-Revillet, HBalestrino, DLoh, EGripenland, JTiensuu, TVaitkevicius, KBarthelemy, MVergassola, MNahori, MSoubigou, GRégnault, BCoppée, JLecuit, MJohansson, JCossart, PThe bacterium Listeria monocytogenes is ubiquitous in the environment and can lead to severe food-borne infections. It has recently emerged as a multifaceted model in pathogenesis. However, how this bacterium switches from a saprophyte to a pathogen is largely unknown. Here, using tiling arrays and RNAs from wild-type and mutant bacteria grown in vitro, ex vivo and in vivo, we have analysed the transcription of its entire genome. We provide the complete Listeria operon map and have uncovered far more diverse types of RNAs than expected: in addition to 50 small RNAs (<500 nucleotides), at least two of which are involved in virulence in mice, we have identified antisense RNAs covering several open-reading frames and long overlapping 5' and 3' untranslated regions. We discovered that riboswitches can act as terminators for upstream genes. When Listeria reaches the host intestinal lumen, an extensive transcriptional reshaping occurs with a SigB-mediated activation of virulence genes. In contrast, in the blood, PrfA controls transcription of virulence genes. Remarkably, several non-coding RNAs absent in the non-pathogenic species Listeria innocua exhibit the same expression patterns as the virulence genes. Together, our data unravel successive and coordinated global transcriptional changes during infection and point to previously unknown regulatory mechanisms in bacteria. |
| spellingShingle | Toledo-Arana, A Dussurget, O Nikitas, G Sesto, N Guet-Revillet, H Balestrino, D Loh, E Gripenland, J Tiensuu, T Vaitkevicius, K Barthelemy, M Vergassola, M Nahori, M Soubigou, G Régnault, B Coppée, J Lecuit, M Johansson, J Cossart, P The Listeria transcriptional landscape from saprophytism to virulence. |
| title | The Listeria transcriptional landscape from saprophytism to virulence. |
| title_full | The Listeria transcriptional landscape from saprophytism to virulence. |
| title_fullStr | The Listeria transcriptional landscape from saprophytism to virulence. |
| title_full_unstemmed | The Listeria transcriptional landscape from saprophytism to virulence. |
| title_short | The Listeria transcriptional landscape from saprophytism to virulence. |
| title_sort | listeria transcriptional landscape from saprophytism to virulence |
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