PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development
Upon fertilisation by sperm, mammalian eggs are activated by a series of intracellular Ca2+ oscillations that are essential for embryo development. The mechanism by which sperm induces this complex signalling phenomenon is unknown. One proposal is that the sperm introduces an exclusive cytosolic fac...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2002
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| _version_ | 1826269549294518272 |
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| author | Saunders, C Larman, MG Parrington, J Cox, L Royse, J Blayney, L Swann, K Lai, F |
| author_facet | Saunders, C Larman, MG Parrington, J Cox, L Royse, J Blayney, L Swann, K Lai, F |
| author_sort | Saunders, C |
| collection | OXFORD |
| description | Upon fertilisation by sperm, mammalian eggs are activated by a series of intracellular Ca2+ oscillations that are essential for embryo development. The mechanism by which sperm induces this complex signalling phenomenon is unknown. One proposal is that the sperm introduces an exclusive cytosolic factor into the egg that elicits serial Ca2+ release. The 'sperm factor' hypothesis has not been ratified because a sperm-specific protein that generates repetitive Ca2+ transients and egg activation has not been found. We identify a novel, sperm-specific phospholipase C, PLCζ, that triggers Ca2+ oscillations in mouse eggs indistinguishable from those at fertilisation. PLCζ removal from sperm extracts abolishes Ca2+ release in eggs. Moreover, the PLCζ content of a single sperm was sufficient to produce Ca2+ oscillations as well as normal embryo development to blastocyst. Our results are consistent with sperm PLCζ as the molecular trigger for development of a fertilised egg into an embryo. |
| first_indexed | 2024-03-06T21:26:47Z |
| format | Journal article |
| id | oxford-uuid:435ffb6e-f070-4545-b801-5f60123179e6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:26:47Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:435ffb6e-f070-4545-b801-5f60123179e62022-03-26T14:54:58ZPLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo developmentJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:435ffb6e-f070-4545-b801-5f60123179e6EnglishSymplectic Elements at Oxford2002Saunders, CLarman, MGParrington, JCox, LRoyse, JBlayney, LSwann, KLai, FUpon fertilisation by sperm, mammalian eggs are activated by a series of intracellular Ca2+ oscillations that are essential for embryo development. The mechanism by which sperm induces this complex signalling phenomenon is unknown. One proposal is that the sperm introduces an exclusive cytosolic factor into the egg that elicits serial Ca2+ release. The 'sperm factor' hypothesis has not been ratified because a sperm-specific protein that generates repetitive Ca2+ transients and egg activation has not been found. We identify a novel, sperm-specific phospholipase C, PLCζ, that triggers Ca2+ oscillations in mouse eggs indistinguishable from those at fertilisation. PLCζ removal from sperm extracts abolishes Ca2+ release in eggs. Moreover, the PLCζ content of a single sperm was sufficient to produce Ca2+ oscillations as well as normal embryo development to blastocyst. Our results are consistent with sperm PLCζ as the molecular trigger for development of a fertilised egg into an embryo. |
| spellingShingle | Saunders, C Larman, MG Parrington, J Cox, L Royse, J Blayney, L Swann, K Lai, F PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development |
| title | PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development |
| title_full | PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development |
| title_fullStr | PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development |
| title_full_unstemmed | PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development |
| title_short | PLCζ: A sperm-specific trigger of Ca2+ oscillations in eggs and embryo development |
| title_sort | plcζ a sperm specific trigger of ca2 oscillations in eggs and embryo development |
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