Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro.
Hepatitis C virus (HCV) is a major cause of liver disease but the full impact of HCV infection on the hepatocyte is poorly understood. RNA sequencing (RNA-Seq) is a novel method to analyze the full transcriptional activity of a cell or tissue, thus allowing new insight into the impact of HCV infecti...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Wiley
2010
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| _version_ | 1797065408974422016 |
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| author | Woodhouse, S Narayan, R Latham, S Lee, S Antrobus, R Gangadharan, B Luo, S Schroth, G Klenerman, P Zitzmann, N |
| author_facet | Woodhouse, S Narayan, R Latham, S Lee, S Antrobus, R Gangadharan, B Luo, S Schroth, G Klenerman, P Zitzmann, N |
| author_sort | Woodhouse, S |
| collection | OXFORD |
| description | Hepatitis C virus (HCV) is a major cause of liver disease but the full impact of HCV infection on the hepatocyte is poorly understood. RNA sequencing (RNA-Seq) is a novel method to analyze the full transcriptional activity of a cell or tissue, thus allowing new insight into the impact of HCV infection. We conducted the first full-genome RNA-Seq analysis in a host cell to analyze infected and noninfected cells, and compared this to microarray and proteomic analyses. The combined power of the triple approach revealed that HCV infection affects a number of previously unreported canonical pathways and biological functions, including pregnane X receptor/retinoic acid receptor activation as a potential host antiviral response, and integrin-linked kinase signaling as an entry factor. This approach also identified several mechanisms implicated in HCV pathogenesis, including an increase in reactive oxygen species. HCV infection had a broad effect on cellular metabolism, leading to increases in cellular cholesterol and free fatty acid levels, associated with a profound and specific decrease in cellular glucose levels.RNA-Seq technology, especially when combined with established methods, demonstrated that HCV infection has potentially wide-ranging effects on cellular gene and protein expression. This in vitro study indicates a substantial metabolic impact of HCV infection and highlights new mechanisms of virus-host interaction which may be highly relevant to pathogenesis in vivo. |
| first_indexed | 2024-03-06T21:28:15Z |
| format | Journal article |
| id | oxford-uuid:43d1df2a-9020-4074-bf51-0af0277c37d3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:28:15Z |
| publishDate | 2010 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:43d1df2a-9020-4074-bf51-0af0277c37d32022-03-26T14:57:47ZTranscriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:43d1df2a-9020-4074-bf51-0af0277c37d3EnglishSymplectic Elements at OxfordWiley2010Woodhouse, SNarayan, RLatham, SLee, SAntrobus, RGangadharan, BLuo, SSchroth, GKlenerman, PZitzmann, NHepatitis C virus (HCV) is a major cause of liver disease but the full impact of HCV infection on the hepatocyte is poorly understood. RNA sequencing (RNA-Seq) is a novel method to analyze the full transcriptional activity of a cell or tissue, thus allowing new insight into the impact of HCV infection. We conducted the first full-genome RNA-Seq analysis in a host cell to analyze infected and noninfected cells, and compared this to microarray and proteomic analyses. The combined power of the triple approach revealed that HCV infection affects a number of previously unreported canonical pathways and biological functions, including pregnane X receptor/retinoic acid receptor activation as a potential host antiviral response, and integrin-linked kinase signaling as an entry factor. This approach also identified several mechanisms implicated in HCV pathogenesis, including an increase in reactive oxygen species. HCV infection had a broad effect on cellular metabolism, leading to increases in cellular cholesterol and free fatty acid levels, associated with a profound and specific decrease in cellular glucose levels.RNA-Seq technology, especially when combined with established methods, demonstrated that HCV infection has potentially wide-ranging effects on cellular gene and protein expression. This in vitro study indicates a substantial metabolic impact of HCV infection and highlights new mechanisms of virus-host interaction which may be highly relevant to pathogenesis in vivo. |
| spellingShingle | Woodhouse, S Narayan, R Latham, S Lee, S Antrobus, R Gangadharan, B Luo, S Schroth, G Klenerman, P Zitzmann, N Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro. |
| title | Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro. |
| title_full | Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro. |
| title_fullStr | Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro. |
| title_full_unstemmed | Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro. |
| title_short | Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro. |
| title_sort | transcriptome sequencing microarray and proteomic analyses reveal cellular and metabolic impact of hepatitis c virus infection in vitro |
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