Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans
Background: A high dietary protein intake has been shown to blunt the deposition of intrahepatic lipids in high-fat- and high-carbohydrate-fed rodents and humans. Objective: The aim of this study was to evaluate the effect of essential amino acid supplementation on the increase in hepatic fat conten...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Sprog: | English |
| Udgivet: |
2012
|
| _version_ | 1826269659605762048 |
|---|---|
| author | Theytaz, F Noguchi, Y Egli, L Campos, V Buehler, T Hodson, L Patterson, B Nishikata, N Kreis, R Mittendorfer, B Fielding, B Boesch, C Tappy, L |
| author_facet | Theytaz, F Noguchi, Y Egli, L Campos, V Buehler, T Hodson, L Patterson, B Nishikata, N Kreis, R Mittendorfer, B Fielding, B Boesch, C Tappy, L |
| author_sort | Theytaz, F |
| collection | OXFORD |
| description | Background: A high dietary protein intake has been shown to blunt the deposition of intrahepatic lipids in high-fat- and high-carbohydrate-fed rodents and humans. Objective: The aim of this study was to evaluate the effect of essential amino acid supplementation on the increase in hepatic fat content induced by a high-fructose diet in healthy subjects. Design: Nine healthy male volunteers were studied on 3 occasions in a randomized, crossover design after 6 d of dietary intervention. Dietary conditions consisted of a weight-maintenance balanced diet (control) or the same balanced diet supplemented with 3 g fructose·kg-1·d-1and 6.77 g of a mixture of 5 essential amino acids 3 times/d (leucine, isoleucine, valine, lysine, and threonine) (HFrAA) or with 3 g fructose·kg-1·d -1 and a maltodextrin placebo 3 times/d (HFr); there was a washout period of 4 to 10 wk between each condition. For each condition, the intrahepatocellular lipid (IHCL) concentration, VLDL-triglyceride concentration, and VLDL-[13C]palmitate production were measured after oral loading with [13C]fructose. Results: HFr increased the IHCL content (1.27 ± 0.31 compared with 2.74 ± 0.55 vol %; P < 0.05) and VLDL-triglyceride (0.55 ± 0.06 compared with 1.40 ± 0.15 mmol/L; P < 0.05). HFr also enhanced VLDL-[13C]palmitate production. HFrAA significantly decreased IHCL compared with HFr (to 2.30 ± 0.43 vol%; P < 0.05) but did not change VLDL-triglyceride concentrations or VLDL-[ 13C]palmitate production. Conclusions: Supplementation with essential amino acids blunts the fructose-induced increase in IHCL but not hypertriglyceridemia. This is not because of inhibition of VLDL-[ 13C]palmitate production. This trial was registered at www.clinicaltrials.gov as NCT01119989. © 2012 American Society for Nutrition. |
| first_indexed | 2024-03-06T21:28:33Z |
| format | Journal article |
| id | oxford-uuid:43e7737f-fbf6-4e2b-aca3-264cd31b86d6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:28:33Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:43e7737f-fbf6-4e2b-aca3-264cd31b86d62022-03-26T14:58:24ZEffects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humansJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:43e7737f-fbf6-4e2b-aca3-264cd31b86d6EnglishSymplectic Elements at Oxford2012Theytaz, FNoguchi, YEgli, LCampos, VBuehler, THodson, LPatterson, BNishikata, NKreis, RMittendorfer, BFielding, BBoesch, CTappy, LBackground: A high dietary protein intake has been shown to blunt the deposition of intrahepatic lipids in high-fat- and high-carbohydrate-fed rodents and humans. Objective: The aim of this study was to evaluate the effect of essential amino acid supplementation on the increase in hepatic fat content induced by a high-fructose diet in healthy subjects. Design: Nine healthy male volunteers were studied on 3 occasions in a randomized, crossover design after 6 d of dietary intervention. Dietary conditions consisted of a weight-maintenance balanced diet (control) or the same balanced diet supplemented with 3 g fructose·kg-1·d-1and 6.77 g of a mixture of 5 essential amino acids 3 times/d (leucine, isoleucine, valine, lysine, and threonine) (HFrAA) or with 3 g fructose·kg-1·d -1 and a maltodextrin placebo 3 times/d (HFr); there was a washout period of 4 to 10 wk between each condition. For each condition, the intrahepatocellular lipid (IHCL) concentration, VLDL-triglyceride concentration, and VLDL-[13C]palmitate production were measured after oral loading with [13C]fructose. Results: HFr increased the IHCL content (1.27 ± 0.31 compared with 2.74 ± 0.55 vol %; P < 0.05) and VLDL-triglyceride (0.55 ± 0.06 compared with 1.40 ± 0.15 mmol/L; P < 0.05). HFr also enhanced VLDL-[13C]palmitate production. HFrAA significantly decreased IHCL compared with HFr (to 2.30 ± 0.43 vol%; P < 0.05) but did not change VLDL-triglyceride concentrations or VLDL-[ 13C]palmitate production. Conclusions: Supplementation with essential amino acids blunts the fructose-induced increase in IHCL but not hypertriglyceridemia. This is not because of inhibition of VLDL-[ 13C]palmitate production. This trial was registered at www.clinicaltrials.gov as NCT01119989. © 2012 American Society for Nutrition. |
| spellingShingle | Theytaz, F Noguchi, Y Egli, L Campos, V Buehler, T Hodson, L Patterson, B Nishikata, N Kreis, R Mittendorfer, B Fielding, B Boesch, C Tappy, L Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans |
| title | Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans |
| title_full | Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans |
| title_fullStr | Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans |
| title_full_unstemmed | Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans |
| title_short | Effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans |
| title_sort | effects of supplementation with essential amino acids on intrahepatic lipid concentrations during fructose overfeeding in humans |
| work_keys_str_mv | AT theytazf effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT noguchiy effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT eglil effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT camposv effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT buehlert effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT hodsonl effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT pattersonb effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT nishikatan effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT kreisr effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT mittendorferb effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT fieldingb effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT boeschc effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans AT tappyl effectsofsupplementationwithessentialaminoacidsonintrahepaticlipidconcentrationsduringfructoseoverfeedinginhumans |