Rapid effector function in CD8+ memory T cells.
The nature of the CD8+ T cells that underlie antiviral protective immunological memory in vivo is unclear. We have characterized peptide-specific CD8+ T lymphocytes directly ex vivo from peripheral blood in humans with past exposure to influenza virus, using single cell interferon gamma (IFN-gamma)...
| Huvudupphovsmän: | , , , , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
1997
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| _version_ | 1826269681608032256 |
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| author | Lalvani, A Brookes, R Hambleton, S Britton, W Hill, A McMichael, A |
| author_facet | Lalvani, A Brookes, R Hambleton, S Britton, W Hill, A McMichael, A |
| author_sort | Lalvani, A |
| collection | OXFORD |
| description | The nature of the CD8+ T cells that underlie antiviral protective immunological memory in vivo is unclear. We have characterized peptide-specific CD8+ T lymphocytes directly ex vivo from peripheral blood in humans with past exposure to influenza virus, using single cell interferon gamma (IFN-gamma) release as a measure of effector function. In individuals in the memory state with respect to influenza virus infection, unrestimulated antigen-specific CD8+ T cells displayed IFN-gamma release within 6 h of antigen contact, identifying a population of memory CD8+ T cells that exhibit effector function without needing to divide and differentiate over several days. We have quantified circulating CD8+ effector T cells specific for six different MHC class I-restricted influenza virus epitopes. Enumeration of these CD8+ T cells gives frequencies of peptide-specific T cells that correlate with, but are in general severalfold higher than, CTL precursor frequencies derived from limiting dilution analysis, indicating that this novel population of memory CD8+ T cells has hitherto been undetected by standard means. The phenotype of these cells, which persist at a low frequency long after recovery from an acute viral infection, suggests that they play a role in protective immunological memory. |
| first_indexed | 2024-03-06T21:28:54Z |
| format | Journal article |
| id | oxford-uuid:44005c81-7182-4f11-ac0c-b11c1a28b25a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:28:54Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:44005c81-7182-4f11-ac0c-b11c1a28b25a2022-03-26T14:59:01ZRapid effector function in CD8+ memory T cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:44005c81-7182-4f11-ac0c-b11c1a28b25aEnglishSymplectic Elements at Oxford1997Lalvani, ABrookes, RHambleton, SBritton, WHill, AMcMichael, AThe nature of the CD8+ T cells that underlie antiviral protective immunological memory in vivo is unclear. We have characterized peptide-specific CD8+ T lymphocytes directly ex vivo from peripheral blood in humans with past exposure to influenza virus, using single cell interferon gamma (IFN-gamma) release as a measure of effector function. In individuals in the memory state with respect to influenza virus infection, unrestimulated antigen-specific CD8+ T cells displayed IFN-gamma release within 6 h of antigen contact, identifying a population of memory CD8+ T cells that exhibit effector function without needing to divide and differentiate over several days. We have quantified circulating CD8+ effector T cells specific for six different MHC class I-restricted influenza virus epitopes. Enumeration of these CD8+ T cells gives frequencies of peptide-specific T cells that correlate with, but are in general severalfold higher than, CTL precursor frequencies derived from limiting dilution analysis, indicating that this novel population of memory CD8+ T cells has hitherto been undetected by standard means. The phenotype of these cells, which persist at a low frequency long after recovery from an acute viral infection, suggests that they play a role in protective immunological memory. |
| spellingShingle | Lalvani, A Brookes, R Hambleton, S Britton, W Hill, A McMichael, A Rapid effector function in CD8+ memory T cells. |
| title | Rapid effector function in CD8+ memory T cells. |
| title_full | Rapid effector function in CD8+ memory T cells. |
| title_fullStr | Rapid effector function in CD8+ memory T cells. |
| title_full_unstemmed | Rapid effector function in CD8+ memory T cells. |
| title_short | Rapid effector function in CD8+ memory T cells. |
| title_sort | rapid effector function in cd8 memory t cells |
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