Dicer-dependent turnover of intergenic transcripts from the human beta-globin gene cluster.
The widespread occurrence of intergenic transcription in eukaryotes is increasingly evident. Intergenic transcription in the beta-globin gene cluster has been described in murine and human cells, and models for a role in gene and chromatin activation have been proposed. In this study, we analyze int...
| Main Authors: | , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2005
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| _version_ | 1826269722123960320 |
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| author | Haussecker, D Proudfoot, N |
| author_facet | Haussecker, D Proudfoot, N |
| author_sort | Haussecker, D |
| collection | OXFORD |
| description | The widespread occurrence of intergenic transcription in eukaryotes is increasingly evident. Intergenic transcription in the beta-globin gene cluster has been described in murine and human cells, and models for a role in gene and chromatin activation have been proposed. In this study, we analyze intergenic transcription and the chromatin state throughout the human beta-globin gene cluster and find that the data are not consistent with such activation-linked models. Thus, intergenic transcript levels correlate with neither chromatin activation nor globin gene expression. Instead, we find that intergenic transcripts of the beta-globin gene cluster are specifically upregulated in Dicer-deficient cells. This is accompanied by a shift towards more activated chromatin as indicated by changes in histone tail modifications. Our results strongly implicate RNA interference (RNAi)-related mechanisms in regulating intergenic transcription in the human beta-globin gene cluster and further suggest that RNAi-dependent chromatin silencing in vertebrates is not restricted to the centromeres. |
| first_indexed | 2024-03-06T21:29:33Z |
| format | Journal article |
| id | oxford-uuid:443649b4-7511-4a36-baf4-bd040fb8d223 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:29:33Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:443649b4-7511-4a36-baf4-bd040fb8d2232022-03-26T15:00:11ZDicer-dependent turnover of intergenic transcripts from the human beta-globin gene cluster.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:443649b4-7511-4a36-baf4-bd040fb8d223EnglishSymplectic Elements at Oxford2005Haussecker, DProudfoot, NThe widespread occurrence of intergenic transcription in eukaryotes is increasingly evident. Intergenic transcription in the beta-globin gene cluster has been described in murine and human cells, and models for a role in gene and chromatin activation have been proposed. In this study, we analyze intergenic transcription and the chromatin state throughout the human beta-globin gene cluster and find that the data are not consistent with such activation-linked models. Thus, intergenic transcript levels correlate with neither chromatin activation nor globin gene expression. Instead, we find that intergenic transcripts of the beta-globin gene cluster are specifically upregulated in Dicer-deficient cells. This is accompanied by a shift towards more activated chromatin as indicated by changes in histone tail modifications. Our results strongly implicate RNA interference (RNAi)-related mechanisms in regulating intergenic transcription in the human beta-globin gene cluster and further suggest that RNAi-dependent chromatin silencing in vertebrates is not restricted to the centromeres. |
| spellingShingle | Haussecker, D Proudfoot, N Dicer-dependent turnover of intergenic transcripts from the human beta-globin gene cluster. |
| title | Dicer-dependent turnover of intergenic transcripts from the human beta-globin gene cluster. |
| title_full | Dicer-dependent turnover of intergenic transcripts from the human beta-globin gene cluster. |
| title_fullStr | Dicer-dependent turnover of intergenic transcripts from the human beta-globin gene cluster. |
| title_full_unstemmed | Dicer-dependent turnover of intergenic transcripts from the human beta-globin gene cluster. |
| title_short | Dicer-dependent turnover of intergenic transcripts from the human beta-globin gene cluster. |
| title_sort | dicer dependent turnover of intergenic transcripts from the human beta globin gene cluster |
| work_keys_str_mv | AT hausseckerd dicerdependentturnoverofintergenictranscriptsfromthehumanbetaglobingenecluster AT proudfootn dicerdependentturnoverofintergenictranscriptsfromthehumanbetaglobingenecluster |