A method for generating single metalloprotein crystals in well-defined redox states: combined electrochemical control and infrared microspectroscopic imaging of a NiFe hydrogenase crystal
We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchtroton infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electroc...
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Bibliographic Details
| Main Authors: |
Ash, P,
Carr, S,
Reeve, H,
Rowbotham, J,
Shutt, R,
Frogley, M,
Evans, R,
Cinque, G,
Armstrong, F,
Vincent, K |
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| Format: | Journal article
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| Published: |
Royal Society of Chemistry
2017
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