A method for generating single metalloprotein crystals in well-defined redox states: combined electrochemical control and infrared microspectroscopic imaging of a NiFe hydrogenase crystal

A method for generating single metalloprotein crystals in well-defined redox states: combined electrochemical control and infrared microspectroscopic imaging of a NiFe hydrogenase crystal

We describe an approach to generating and verifying well-defined redox states in metalloprotein single crystals by combining electrochemical control with synchtroton infrared microspectroscopic imaging. For NiFe hydrogenase 1 from Escherichia coli we demonstrate fully reversible and uniform electroc...

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Bibliographic Details
Main Authors:	Ash, P, Carr, S, Reeve, H, Rowbotham, J, Shutt, R, Frogley, M, Evans, R, Cinque, G, Armstrong, F, Vincent, K
Format:	Journal article
Published:	Royal Society of Chemistry 2017

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