X-ray radiation damage to biological macromolecules: further insights
Despite significant progress made over more than 15 years of research, structural biologists are still grappling with the issue of radiation damage suffered by macromolecular crystals which is induced by the resultant radiation chemistry occurring during X-ray diffraction experiments. Further insigh...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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International Union of Crystallography
2017
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| _version_ | 1797065616410017792 |
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| author | Garman, E Weik, M |
| author_facet | Garman, E Weik, M |
| author_sort | Garman, E |
| collection | OXFORD |
| description | Despite significant progress made over more than 15 years of research, structural biologists are still grappling with the issue of radiation damage suffered by macromolecular crystals which is induced by the resultant radiation chemistry occurring during X-ray diffraction experiments. Further insights into these effects and the possible mitigation strategies for use in both diffraction and SAXS experiments are given in eight papers in this volume. In particular, damage during experimental phasing is addressed, scavengers for SAXS experiments are investigated, microcrystals are imaged, data collection strategies are optimized, specific damage to tyrosine residues is reexamined, and room temperature conformational heterogeneity as a function of dose is explored. The brief summary below puts these papers into perspective relative to other ongoing radiation damage research on macromolecules. |
| first_indexed | 2024-03-06T21:31:11Z |
| format | Journal article |
| id | oxford-uuid:44bbe05d-2548-4e9a-b1d4-b5d744fbc6bd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:31:11Z |
| publishDate | 2017 |
| publisher | International Union of Crystallography |
| record_format | dspace |
| spelling | oxford-uuid:44bbe05d-2548-4e9a-b1d4-b5d744fbc6bd2022-03-26T15:03:26ZX-ray radiation damage to biological macromolecules: further insightsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:44bbe05d-2548-4e9a-b1d4-b5d744fbc6bdEnglishSymplectic Elements at OxfordInternational Union of Crystallography2017Garman, EWeik, MDespite significant progress made over more than 15 years of research, structural biologists are still grappling with the issue of radiation damage suffered by macromolecular crystals which is induced by the resultant radiation chemistry occurring during X-ray diffraction experiments. Further insights into these effects and the possible mitigation strategies for use in both diffraction and SAXS experiments are given in eight papers in this volume. In particular, damage during experimental phasing is addressed, scavengers for SAXS experiments are investigated, microcrystals are imaged, data collection strategies are optimized, specific damage to tyrosine residues is reexamined, and room temperature conformational heterogeneity as a function of dose is explored. The brief summary below puts these papers into perspective relative to other ongoing radiation damage research on macromolecules. |
| spellingShingle | Garman, E Weik, M X-ray radiation damage to biological macromolecules: further insights |
| title | X-ray radiation damage to biological macromolecules: further insights |
| title_full | X-ray radiation damage to biological macromolecules: further insights |
| title_fullStr | X-ray radiation damage to biological macromolecules: further insights |
| title_full_unstemmed | X-ray radiation damage to biological macromolecules: further insights |
| title_short | X-ray radiation damage to biological macromolecules: further insights |
| title_sort | x ray radiation damage to biological macromolecules further insights |
| work_keys_str_mv | AT garmane xrayradiationdamagetobiologicalmacromoleculesfurtherinsights AT weikm xrayradiationdamagetobiologicalmacromoleculesfurtherinsights |