Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera)
By means of a tracer assay using a labeled synthetic angiotensin converting enzyme (ACE) substrate hippurylglycylglycine, we have detected high ACE activity in the testes of the African migratory locust, Locusta migratoria. Lower, but significant, ACE activity was observed in midgut and hemolymph. I...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2003
|
| _version_ | 1826269907465011200 |
|---|---|
| author | Macours, N Vandingenen, A Gielens, C Hens, K Baggerman, G Schoofs, L Huybrechts, R |
| author_facet | Macours, N Vandingenen, A Gielens, C Hens, K Baggerman, G Schoofs, L Huybrechts, R |
| author_sort | Macours, N |
| collection | OXFORD |
| description | By means of a tracer assay using a labeled synthetic angiotensin converting enzyme (ACE) substrate hippurylglycylglycine, we have detected high ACE activity in the testes of the African migratory locust, Locusta migratoria. Lower, but significant, ACE activity was observed in midgut and hemolymph. In a two-step purification procedure involving anion exchange and gel permeation chromatography, we have purified LomACE from the locust testes. The enzyme of approximately 80 kDa shows substantial amino-acid sequence homology with ACE from both vertebrate and invertebrate origin. The ACE identity of the purified enzyme was further confirmed by cDNA cloning of the Locusta ACE fragment, which, after in silico translation, revealed a mature protein of 623 amino acids with a large structural similarity to other known ACE proteins. |
| first_indexed | 2024-03-06T21:32:33Z |
| format | Journal article |
| id | oxford-uuid:45277b3d-6abb-4ff1-982c-b19185cfbea2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:32:33Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:45277b3d-6abb-4ff1-982c-b19185cfbea22022-03-26T15:06:07ZIsolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera)Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:45277b3d-6abb-4ff1-982c-b19185cfbea2EnglishSymplectic Elements at Oxford2003Macours, NVandingenen, AGielens, CHens, KBaggerman, GSchoofs, LHuybrechts, RBy means of a tracer assay using a labeled synthetic angiotensin converting enzyme (ACE) substrate hippurylglycylglycine, we have detected high ACE activity in the testes of the African migratory locust, Locusta migratoria. Lower, but significant, ACE activity was observed in midgut and hemolymph. In a two-step purification procedure involving anion exchange and gel permeation chromatography, we have purified LomACE from the locust testes. The enzyme of approximately 80 kDa shows substantial amino-acid sequence homology with ACE from both vertebrate and invertebrate origin. The ACE identity of the purified enzyme was further confirmed by cDNA cloning of the Locusta ACE fragment, which, after in silico translation, revealed a mature protein of 623 amino acids with a large structural similarity to other known ACE proteins. |
| spellingShingle | Macours, N Vandingenen, A Gielens, C Hens, K Baggerman, G Schoofs, L Huybrechts, R Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera) |
| title | Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera) |
| title_full | Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera) |
| title_fullStr | Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera) |
| title_full_unstemmed | Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera) |
| title_short | Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera) |
| title_sort | isolation of angiotensin converting enzyme from testes of locusta migratoria orthoptera |
| work_keys_str_mv | AT macoursn isolationofangiotensinconvertingenzymefromtestesoflocustamigratoriaorthoptera AT vandingenena isolationofangiotensinconvertingenzymefromtestesoflocustamigratoriaorthoptera AT gielensc isolationofangiotensinconvertingenzymefromtestesoflocustamigratoriaorthoptera AT hensk isolationofangiotensinconvertingenzymefromtestesoflocustamigratoriaorthoptera AT baggermang isolationofangiotensinconvertingenzymefromtestesoflocustamigratoriaorthoptera AT schoofsl isolationofangiotensinconvertingenzymefromtestesoflocustamigratoriaorthoptera AT huybrechtsr isolationofangiotensinconvertingenzymefromtestesoflocustamigratoriaorthoptera |