Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels.

The ability to move from the primary infection site and colonize distant tissue in the leaf is an important property of bacterial plant pathogens, yet this aspect has hardly been investigated for model pathogens. Here we show that GFP-expressing Pseudomonas syringae pv. syringae DC3000 that lacks th...

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Main Authors: Misas-Villamil, J, Kolodziejek, I, van der Hoorn, R
Format: Journal article
Language:English
Published: 2011
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author Misas-Villamil, J
Kolodziejek, I
van der Hoorn, R
author_facet Misas-Villamil, J
Kolodziejek, I
van der Hoorn, R
author_sort Misas-Villamil, J
collection OXFORD
description The ability to move from the primary infection site and colonize distant tissue in the leaf is an important property of bacterial plant pathogens, yet this aspect has hardly been investigated for model pathogens. Here we show that GFP-expressing Pseudomonas syringae pv. syringae DC3000 that lacks the HopQ1-1 effector (PtoDC3000ΔhQ) has a strong capacity to colonize distant leaf tissue from wound-inoculated sites in N. benthamiana. Distant colonization occurs within 1 week after toothpick inoculation and is characterized by distant colonies in the apoplast along the vasculature. Distant colonization is blocked by the non-host resistance response triggered by HopQ1-1 in an SGT1-dependent manner and is associated with an explosive growth of the bacterial population, and displays robust growth differences between compatible and incompatible interactions. Scanning electron microscopy revealed that PtoDC3000ΔhQ bacteria are present in xylem vessels, indicating that they use the xylem to move through the leaf blade. Distant colonization does not require flagellin-mediated motility, and is common for P. syringae pathovars that represent different phylogroups.
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spelling oxford-uuid:458b48e5-3cda-473b-adb5-64d19e2d95a82022-03-26T15:08:22ZPseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:458b48e5-3cda-473b-adb5-64d19e2d95a8EnglishSymplectic Elements at Oxford2011Misas-Villamil, JKolodziejek, Ivan der Hoorn, RThe ability to move from the primary infection site and colonize distant tissue in the leaf is an important property of bacterial plant pathogens, yet this aspect has hardly been investigated for model pathogens. Here we show that GFP-expressing Pseudomonas syringae pv. syringae DC3000 that lacks the HopQ1-1 effector (PtoDC3000ΔhQ) has a strong capacity to colonize distant leaf tissue from wound-inoculated sites in N. benthamiana. Distant colonization occurs within 1 week after toothpick inoculation and is characterized by distant colonies in the apoplast along the vasculature. Distant colonization is blocked by the non-host resistance response triggered by HopQ1-1 in an SGT1-dependent manner and is associated with an explosive growth of the bacterial population, and displays robust growth differences between compatible and incompatible interactions. Scanning electron microscopy revealed that PtoDC3000ΔhQ bacteria are present in xylem vessels, indicating that they use the xylem to move through the leaf blade. Distant colonization does not require flagellin-mediated motility, and is common for P. syringae pathovars that represent different phylogroups.
spellingShingle Misas-Villamil, J
Kolodziejek, I
van der Hoorn, R
Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels.
title Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels.
title_full Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels.
title_fullStr Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels.
title_full_unstemmed Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels.
title_short Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels.
title_sort pseudomonas syringae colonizes distant tissues in nicotiana benthamiana through xylem vessels
work_keys_str_mv AT misasvillamilj pseudomonassyringaecolonizesdistanttissuesinnicotianabenthamianathroughxylemvessels
AT kolodziejeki pseudomonassyringaecolonizesdistanttissuesinnicotianabenthamianathroughxylemvessels
AT vanderhoornr pseudomonassyringaecolonizesdistanttissuesinnicotianabenthamianathroughxylemvessels