Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2.
Hypoxia is central to both ischaemic and neoplastic diseases. However, the non-coding transcriptional response to hypoxia is largely uncharacterized. We undertook integrated genomic analyses of both non-coding and coding transcripts using massively parallel sequencing and interfaced this data with p...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2014
|
| _version_ | 1797065880106958848 |
|---|---|
| author | Choudhry, H Schödel, J Oikonomopoulos, S Camps, C Grampp, S Harris, A Ratcliffe, P Ragoussis, J Mole, D |
| author_facet | Choudhry, H Schödel, J Oikonomopoulos, S Camps, C Grampp, S Harris, A Ratcliffe, P Ragoussis, J Mole, D |
| author_sort | Choudhry, H |
| collection | OXFORD |
| description | Hypoxia is central to both ischaemic and neoplastic diseases. However, the non-coding transcriptional response to hypoxia is largely uncharacterized. We undertook integrated genomic analyses of both non-coding and coding transcripts using massively parallel sequencing and interfaced this data with pan-genomic analyses of hypoxia-inducible factor (HIF) and RNApol2 binding in hypoxic cells. These analyses revealed that all classes of RNA are profoundly regulated by hypoxia and implicated HIF as a major direct regulator of both the non-coding and coding transcriptome, acting predominantly through release of pre-bound promoter-paused RNApol2. These findings indicate that the transcriptional response to hypoxia is substantially more extensive than previously considered. |
| first_indexed | 2024-03-06T21:34:43Z |
| format | Journal article |
| id | oxford-uuid:45d685b7-b48f-42ef-a87c-6cc05f056c3f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:34:43Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:45d685b7-b48f-42ef-a87c-6cc05f056c3f2022-03-26T15:10:15ZExtensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:45d685b7-b48f-42ef-a87c-6cc05f056c3fEnglishSymplectic Elements at Oxford2014Choudhry, HSchödel, JOikonomopoulos, SCamps, CGrampp, SHarris, ARatcliffe, PRagoussis, JMole, DHypoxia is central to both ischaemic and neoplastic diseases. However, the non-coding transcriptional response to hypoxia is largely uncharacterized. We undertook integrated genomic analyses of both non-coding and coding transcripts using massively parallel sequencing and interfaced this data with pan-genomic analyses of hypoxia-inducible factor (HIF) and RNApol2 binding in hypoxic cells. These analyses revealed that all classes of RNA are profoundly regulated by hypoxia and implicated HIF as a major direct regulator of both the non-coding and coding transcriptome, acting predominantly through release of pre-bound promoter-paused RNApol2. These findings indicate that the transcriptional response to hypoxia is substantially more extensive than previously considered. |
| spellingShingle | Choudhry, H Schödel, J Oikonomopoulos, S Camps, C Grampp, S Harris, A Ratcliffe, P Ragoussis, J Mole, D Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2. |
| title | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2. |
| title_full | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2. |
| title_fullStr | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2. |
| title_full_unstemmed | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2. |
| title_short | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2. |
| title_sort | extensive regulation of the non coding transcriptome by hypoxia role of hif in releasing paused rnapol2 |
| work_keys_str_mv | AT choudhryh extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 AT schodelj extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 AT oikonomopouloss extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 AT campsc extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 AT grampps extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 AT harrisa extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 AT ratcliffep extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 AT ragoussisj extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 AT moled extensiveregulationofthenoncodingtranscriptomebyhypoxiaroleofhifinreleasingpausedrnapol2 |