The CRAPome: A contaminant repository for affinity purification-mass spectrometry data
Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for the identification of protein-protein interactions. However, for any given protein of interest, determining which of the identified polypeptides represent bona fide interactors versus those that are background...
| Huvudupphovsmän: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
2013
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| _version_ | 1826270047429984256 |
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| author | Mellacheruvu, D Wright, Z Couzens, A Lambert, J St-Denis, N Li, T Miteva, Y Hauri, S Sardiu, M Low, T Halim, V Bagshaw, R Hubner, N Al-Hakim, A Bouchard, A Faubert, D Fermin, D Dunham, W Goudreault, M Lin, Z Badillo, BG Pawson, T Durocher, D Coulombe, B Aebersold, R |
| author_facet | Mellacheruvu, D Wright, Z Couzens, A Lambert, J St-Denis, N Li, T Miteva, Y Hauri, S Sardiu, M Low, T Halim, V Bagshaw, R Hubner, N Al-Hakim, A Bouchard, A Faubert, D Fermin, D Dunham, W Goudreault, M Lin, Z Badillo, BG Pawson, T Durocher, D Coulombe, B Aebersold, R |
| author_sort | Mellacheruvu, D |
| collection | OXFORD |
| description | Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for the identification of protein-protein interactions. However, for any given protein of interest, determining which of the identified polypeptides represent bona fide interactors versus those that are background contaminants (for example, proteins that interact with the solid-phase support, affinity reagent or epitope tag) is a challenging task. The standard approach is to identify nonspecific interactions using one or more negative-control purifications, but many small-scale AP-MS studies do not capture a complete, accurate background protein set when available controls are limited. Fortunately, negative controls are largely bait independent. Hence, aggregating negative controls from multiple AP-MS studies can increase coverage and improve the characterization of background associated with a given experimental protocol. Here we present the contaminant repository for affinity purification (the CRAPome) and describe its use for scoring protein-protein interactions. The repository (currently available for Homo sapiens and Saccharomyces cerevisiae) and computational tools are freely accessible at http://www.crapome.org/. © 2013 Nature America, Inc. All rights reserved. |
| first_indexed | 2024-03-06T21:34:45Z |
| format | Journal article |
| id | oxford-uuid:45d95c03-f7b8-4091-9091-817b0f11bc9d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:34:45Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:45d95c03-f7b8-4091-9091-817b0f11bc9d2022-03-26T15:10:23ZThe CRAPome: A contaminant repository for affinity purification-mass spectrometry dataJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:45d95c03-f7b8-4091-9091-817b0f11bc9dEnglishSymplectic Elements at Oxford2013Mellacheruvu, DWright, ZCouzens, ALambert, JSt-Denis, NLi, TMiteva, YHauri, SSardiu, MLow, THalim, VBagshaw, RHubner, NAl-Hakim, ABouchard, AFaubert, DFermin, DDunham, WGoudreault, MLin, ZBadillo, BGPawson, TDurocher, DCoulombe, BAebersold, RAffinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for the identification of protein-protein interactions. However, for any given protein of interest, determining which of the identified polypeptides represent bona fide interactors versus those that are background contaminants (for example, proteins that interact with the solid-phase support, affinity reagent or epitope tag) is a challenging task. The standard approach is to identify nonspecific interactions using one or more negative-control purifications, but many small-scale AP-MS studies do not capture a complete, accurate background protein set when available controls are limited. Fortunately, negative controls are largely bait independent. Hence, aggregating negative controls from multiple AP-MS studies can increase coverage and improve the characterization of background associated with a given experimental protocol. Here we present the contaminant repository for affinity purification (the CRAPome) and describe its use for scoring protein-protein interactions. The repository (currently available for Homo sapiens and Saccharomyces cerevisiae) and computational tools are freely accessible at http://www.crapome.org/. © 2013 Nature America, Inc. All rights reserved. |
| spellingShingle | Mellacheruvu, D Wright, Z Couzens, A Lambert, J St-Denis, N Li, T Miteva, Y Hauri, S Sardiu, M Low, T Halim, V Bagshaw, R Hubner, N Al-Hakim, A Bouchard, A Faubert, D Fermin, D Dunham, W Goudreault, M Lin, Z Badillo, BG Pawson, T Durocher, D Coulombe, B Aebersold, R The CRAPome: A contaminant repository for affinity purification-mass spectrometry data |
| title | The CRAPome: A contaminant repository for affinity purification-mass spectrometry data |
| title_full | The CRAPome: A contaminant repository for affinity purification-mass spectrometry data |
| title_fullStr | The CRAPome: A contaminant repository for affinity purification-mass spectrometry data |
| title_full_unstemmed | The CRAPome: A contaminant repository for affinity purification-mass spectrometry data |
| title_short | The CRAPome: A contaminant repository for affinity purification-mass spectrometry data |
| title_sort | crapome a contaminant repository for affinity purification mass spectrometry data |
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