FtsK translocation on DNA stops at XerCD-dif.
Escherichia coli FtsK is a powerful, fast, double-stranded DNA translocase, which can strip proteins from DNA. FtsK acts in the late stages of chromosome segregation by facilitating sister chromosome unlinking at the division septum. KOPS-guided DNA translocation directs FtsK towards dif, located wi...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Oxford University Press
2010
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| _version_ | 1826270094556135424 |
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| author | Graham, J Sivanathan, V Sherratt, D Arciszewska, L |
| author_facet | Graham, J Sivanathan, V Sherratt, D Arciszewska, L |
| author_sort | Graham, J |
| collection | OXFORD |
| description | Escherichia coli FtsK is a powerful, fast, double-stranded DNA translocase, which can strip proteins from DNA. FtsK acts in the late stages of chromosome segregation by facilitating sister chromosome unlinking at the division septum. KOPS-guided DNA translocation directs FtsK towards dif, located within the replication terminus region, ter, where FtsK activates XerCD site-specific recombination. Here we show that FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. Stoppage of translocation at XerCD-dif is accompanied by a reduction in FtsK ATPase and is not associated with FtsK dissociation from DNA. Specific stoppage at recombinase-DNA complexes does not require the FtsKgamma regulatory subdomain, which interacts with XerD, and is not dependent on either recombinase-mediated DNA cleavage activity, or the formation of synaptic complexes. |
| first_indexed | 2024-03-06T21:35:28Z |
| format | Journal article |
| id | oxford-uuid:461b09ca-3d78-4c68-a99c-5dec5c5d568a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:35:28Z |
| publishDate | 2010 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:461b09ca-3d78-4c68-a99c-5dec5c5d568a2022-03-26T15:11:44ZFtsK translocation on DNA stops at XerCD-dif.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:461b09ca-3d78-4c68-a99c-5dec5c5d568aEnglishSymplectic Elements at OxfordOxford University Press2010Graham, JSivanathan, VSherratt, DArciszewska, LEscherichia coli FtsK is a powerful, fast, double-stranded DNA translocase, which can strip proteins from DNA. FtsK acts in the late stages of chromosome segregation by facilitating sister chromosome unlinking at the division septum. KOPS-guided DNA translocation directs FtsK towards dif, located within the replication terminus region, ter, where FtsK activates XerCD site-specific recombination. Here we show that FtsK translocation stops specifically at XerCD-dif, thereby preventing removal of XerCD from dif and allowing activation of chromosome unlinking by recombination. Stoppage of translocation at XerCD-dif is accompanied by a reduction in FtsK ATPase and is not associated with FtsK dissociation from DNA. Specific stoppage at recombinase-DNA complexes does not require the FtsKgamma regulatory subdomain, which interacts with XerD, and is not dependent on either recombinase-mediated DNA cleavage activity, or the formation of synaptic complexes. |
| spellingShingle | Graham, J Sivanathan, V Sherratt, D Arciszewska, L FtsK translocation on DNA stops at XerCD-dif. |
| title | FtsK translocation on DNA stops at XerCD-dif. |
| title_full | FtsK translocation on DNA stops at XerCD-dif. |
| title_fullStr | FtsK translocation on DNA stops at XerCD-dif. |
| title_full_unstemmed | FtsK translocation on DNA stops at XerCD-dif. |
| title_short | FtsK translocation on DNA stops at XerCD-dif. |
| title_sort | ftsk translocation on dna stops at xercd dif |
| work_keys_str_mv | AT grahamj ftsktranslocationondnastopsatxercddif AT sivanathanv ftsktranslocationondnastopsatxercddif AT sherrattd ftsktranslocationondnastopsatxercddif AT arciszewskal ftsktranslocationondnastopsatxercddif |