Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection.
<h4>Background</h4> <p>Plasmodium falciparum and Plasmodium vivax infections compromise dendritic cell (DC) function and expand regulatory T (Treg) cells in both clinical disease (malaria) and experimental human sub-microscopic infection. Conversely, in asymptomatic microscopy-posi...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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BioMed Central
2016
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| _version_ | 1797066007028695040 |
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| author | Kho, S Marfurt, J Handayuni, I Pava, Z Noviyanti, R Kusuma, A Piera, K Burdam, F Kenangalem, E Lampah, D Engwerda, C Poespoprodjo, J Price, R Anstey, N Minigo, G Woodberry, T |
| author_facet | Kho, S Marfurt, J Handayuni, I Pava, Z Noviyanti, R Kusuma, A Piera, K Burdam, F Kenangalem, E Lampah, D Engwerda, C Poespoprodjo, J Price, R Anstey, N Minigo, G Woodberry, T |
| author_sort | Kho, S |
| collection | OXFORD |
| description | <h4>Background</h4> <p>Plasmodium falciparum and Plasmodium vivax infections compromise dendritic cell (DC) function and expand regulatory T (Treg) cells in both clinical disease (malaria) and experimental human sub-microscopic infection. Conversely, in asymptomatic microscopy-positive (patent) P. falciparum or P. vivax infection in endemic areas, blood DC increase or retain HLA-DR expression and Treg cells exhibit reduced activation, suggesting that DC and Treg cells contribute to the control of patent asymptomatic infection. The effect of sub-microscopic (sub-patent) asymptomatic Plasmodium infection on DC and Treg cells in malaria-endemic area residents remains unclear</p> <h4>Methods</h4> <p>In a cross-sectional household survey conducted in Papua, Indonesia, 162 asymptomatic adults were prospectively evaluated for DC and Treg cells using field-based flow cytometry. Of these, 161 individuals (99 %) were assessed retrospectively by polymerase chain reaction (PCR), 19 of whom had sub-microscopic infection with P. falciparum and 15 with sub-microscopic P. vivax infection. Flow cytometric data were re-analysed after re-grouping asymptomatic individuals according to PCR results into negative controls, sub-microscopic and microscopic parasitaemia to examine DC and Treg cell phenotype in sub-microscopic infection.</p> <h4>Results</h4> <p>Asymptomatic adults with sub-microscopic P. falciparum or P. vivax infection had DC HLA-DR expression and Treg cell activation comparable to PCR-negative controls. Sub-microscopic P. falciparum infection was associated with lower peripheral CD4+ T cells and lymphocytes, however sub-microscopic Plasmodium infection had no apparent effect on DC sub-set number or Treg cell frequency.</p> <h4>Conclusions</h4> <p>In contrast to the impairment of DC maturation/function and the activation of Treg cells seen with sub-microscopic parasitaemia in primary experimental human Plasmodium infection, no phenotypic evidence of dysregulation of DC and Treg cells was observed in asymptomatic sub-microscopic Plasmodium infection in Indonesian adults. This is consistent with DC and Treg cells retaining their functional capacity in sub-microscopic asymptomatic infection with P. falciparum or P. vivax in malaria-endemic areas.</p> |
| first_indexed | 2024-03-06T21:36:16Z |
| format | Journal article |
| id | oxford-uuid:4660a51d-da69-4a79-9579-efa6a1ad1588 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:36:16Z |
| publishDate | 2016 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oxford-uuid:4660a51d-da69-4a79-9579-efa6a1ad15882022-03-26T15:13:18ZCharacterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4660a51d-da69-4a79-9579-efa6a1ad1588EnglishSymplectic Elements at OxfordBioMed Central2016Kho, SMarfurt, JHandayuni, IPava, ZNoviyanti, RKusuma, APiera, KBurdam, FKenangalem, ELampah, DEngwerda, CPoespoprodjo, JPrice, RAnstey, NMinigo, GWoodberry, T<h4>Background</h4> <p>Plasmodium falciparum and Plasmodium vivax infections compromise dendritic cell (DC) function and expand regulatory T (Treg) cells in both clinical disease (malaria) and experimental human sub-microscopic infection. Conversely, in asymptomatic microscopy-positive (patent) P. falciparum or P. vivax infection in endemic areas, blood DC increase or retain HLA-DR expression and Treg cells exhibit reduced activation, suggesting that DC and Treg cells contribute to the control of patent asymptomatic infection. The effect of sub-microscopic (sub-patent) asymptomatic Plasmodium infection on DC and Treg cells in malaria-endemic area residents remains unclear</p> <h4>Methods</h4> <p>In a cross-sectional household survey conducted in Papua, Indonesia, 162 asymptomatic adults were prospectively evaluated for DC and Treg cells using field-based flow cytometry. Of these, 161 individuals (99 %) were assessed retrospectively by polymerase chain reaction (PCR), 19 of whom had sub-microscopic infection with P. falciparum and 15 with sub-microscopic P. vivax infection. Flow cytometric data were re-analysed after re-grouping asymptomatic individuals according to PCR results into negative controls, sub-microscopic and microscopic parasitaemia to examine DC and Treg cell phenotype in sub-microscopic infection.</p> <h4>Results</h4> <p>Asymptomatic adults with sub-microscopic P. falciparum or P. vivax infection had DC HLA-DR expression and Treg cell activation comparable to PCR-negative controls. Sub-microscopic P. falciparum infection was associated with lower peripheral CD4+ T cells and lymphocytes, however sub-microscopic Plasmodium infection had no apparent effect on DC sub-set number or Treg cell frequency.</p> <h4>Conclusions</h4> <p>In contrast to the impairment of DC maturation/function and the activation of Treg cells seen with sub-microscopic parasitaemia in primary experimental human Plasmodium infection, no phenotypic evidence of dysregulation of DC and Treg cells was observed in asymptomatic sub-microscopic Plasmodium infection in Indonesian adults. This is consistent with DC and Treg cells retaining their functional capacity in sub-microscopic asymptomatic infection with P. falciparum or P. vivax in malaria-endemic areas.</p> |
| spellingShingle | Kho, S Marfurt, J Handayuni, I Pava, Z Noviyanti, R Kusuma, A Piera, K Burdam, F Kenangalem, E Lampah, D Engwerda, C Poespoprodjo, J Price, R Anstey, N Minigo, G Woodberry, T Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection. |
| title | Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection. |
| title_full | Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection. |
| title_fullStr | Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection. |
| title_full_unstemmed | Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection. |
| title_short | Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection. |
| title_sort | characterization of blood dendritic and regulatory t cells in asymptomatic adults with sub microscopic plasmodium falciparum or plasmodium vivax infection |
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