Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae.
Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by the massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. In this article, we identify determinants of this reassembly...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2008
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| _version_ | 1797066007233167360 |
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| author | Jensen, M Christensen, M Bonven, B Jensen, T |
| author_facet | Jensen, M Christensen, M Bonven, B Jensen, T |
| author_sort | Jensen, M |
| collection | OXFORD |
| description | Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by the massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. In this article, we identify determinants of this reassembly throughout the heat shock protein 104 gene (HSP104) transcription unit. The results show that, although histone H3 lacking amino acids 4-30 of its N-terminal tail (H3Delta4-30) is normally deposited, reassembly of H3Delta4-40 is obliterated with an accompanying sustained transcription. On mutation of the histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redeposition and ongoing transcription is not required to prevent chromatin reassembly. |
| first_indexed | 2024-03-06T21:36:17Z |
| format | Journal article |
| id | oxford-uuid:4660c154-1e23-47b7-8348-91e167e0f57e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:36:17Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:4660c154-1e23-47b7-8348-91e167e0f57e2022-03-26T15:13:16ZRequirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4660c154-1e23-47b7-8348-91e167e0f57eEnglishSymplectic Elements at Oxford2008Jensen, MChristensen, MBonven, BJensen, THeat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by the massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. In this article, we identify determinants of this reassembly throughout the heat shock protein 104 gene (HSP104) transcription unit. The results show that, although histone H3 lacking amino acids 4-30 of its N-terminal tail (H3Delta4-30) is normally deposited, reassembly of H3Delta4-40 is obliterated with an accompanying sustained transcription. On mutation of the histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redeposition and ongoing transcription is not required to prevent chromatin reassembly. |
| spellingShingle | Jensen, M Christensen, M Bonven, B Jensen, T Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae. |
| title | Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae. |
| title_full | Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae. |
| title_fullStr | Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae. |
| title_full_unstemmed | Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae. |
| title_short | Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae. |
| title_sort | requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in saccharomyces cerevisiae |
| work_keys_str_mv | AT jensenm requirementsforchromatinreassemblyduringtranscriptionaldownregulationofaheatshockgeneinsaccharomycescerevisiae AT christensenm requirementsforchromatinreassemblyduringtranscriptionaldownregulationofaheatshockgeneinsaccharomycescerevisiae AT bonvenb requirementsforchromatinreassemblyduringtranscriptionaldownregulationofaheatshockgeneinsaccharomycescerevisiae AT jensent requirementsforchromatinreassemblyduringtranscriptionaldownregulationofaheatshockgeneinsaccharomycescerevisiae |