The LFA-1 I-domain: A transient low affinity binding module for ICAM-1
Lymphocyte function associated antigen 1 (LFA-1) is a heterodimer, composed of an α L-chain and a β 2-chain. The I-domain is inserted in the ctL-chain and contains a binding site for ICAM-1. To study the adhesive interaction between the I-domain and IC...
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| Format: | Journal article |
| Language: | English |
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1996
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| _version_ | 1826270175562825728 |
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| author | Knorr, R Dustin, M |
| author_facet | Knorr, R Dustin, M |
| author_sort | Knorr, R |
| collection | OXFORD |
| description | Lymphocyte function associated antigen 1 (LFA-1) is a heterodimer, composed of an α L-chain and a β 2-chain. The I-domain is inserted in the ctL-chain and contains a binding site for ICAM-1. To study the adhesive interaction between the I-domain and ICAM-I, we constructed a GPIanchored form of the I-domain and expressed it stabily in BHK-cells. In contrast to the whole LFA-1 molecule (Kd=8nM), no interaction between soluble ICAM-1 dimers and I-domain expressing cells could be detected, suggesting that the ICAM-1/I-domain interaction is of low affinity (Kd>33| |
| first_indexed | 2024-03-06T21:36:43Z |
| format | Journal article |
| id | oxford-uuid:4682076d-24b7-4b15-b036-c3a28e29e238 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:36:43Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:4682076d-24b7-4b15-b036-c3a28e29e2382022-03-26T15:14:05ZThe LFA-1 I-domain: A transient low affinity binding module for ICAM-1Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4682076d-24b7-4b15-b036-c3a28e29e238EnglishSymplectic Elements at Oxford1996Knorr, RDustin, MLymphocyte function associated antigen 1 (LFA-1) is a heterodimer, composed of an α L-chain and a β 2-chain. The I-domain is inserted in the ctL-chain and contains a binding site for ICAM-1. To study the adhesive interaction between the I-domain and ICAM-I, we constructed a GPIanchored form of the I-domain and expressed it stabily in BHK-cells. In contrast to the whole LFA-1 molecule (Kd=8nM), no interaction between soluble ICAM-1 dimers and I-domain expressing cells could be detected, suggesting that the ICAM-1/I-domain interaction is of low affinity (Kd>33| |
| spellingShingle | Knorr, R Dustin, M The LFA-1 I-domain: A transient low affinity binding module for ICAM-1 |
| title | The LFA-1 I-domain: A transient low affinity binding module for ICAM-1 |
| title_full | The LFA-1 I-domain: A transient low affinity binding module for ICAM-1 |
| title_fullStr | The LFA-1 I-domain: A transient low affinity binding module for ICAM-1 |
| title_full_unstemmed | The LFA-1 I-domain: A transient low affinity binding module for ICAM-1 |
| title_short | The LFA-1 I-domain: A transient low affinity binding module for ICAM-1 |
| title_sort | lfa 1 i domain a transient low affinity binding module for icam 1 |
| work_keys_str_mv | AT knorrr thelfa1idomainatransientlowaffinitybindingmoduleforicam1 AT dustinm thelfa1idomainatransientlowaffinitybindingmoduleforicam1 AT knorrr lfa1idomainatransientlowaffinitybindingmoduleforicam1 AT dustinm lfa1idomainatransientlowaffinitybindingmoduleforicam1 |