Regulation of IL-18 (IFN-gamma-inducing factor) gene expression.
IL-18 (also known as IFN-gamma-inducing factor), although structurally unrelated to IL-12, shares with it the role of activating NK cells and polarizing T cells toward Th1 cell function. To understand how the IL-18 gene (and consequently Th1 function) is regulated, we have determined the gene struct...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1997
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| _version_ | 1826270291617120256 |
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| author | Tone, M Thompson, SA Tone, Y Fairchild, P Waldmann, H |
| author_facet | Tone, M Thompson, SA Tone, Y Fairchild, P Waldmann, H |
| author_sort | Tone, M |
| collection | OXFORD |
| description | IL-18 (also known as IFN-gamma-inducing factor), although structurally unrelated to IL-12, shares with it the role of activating NK cells and polarizing T cells toward Th1 cell function. To understand how the IL-18 gene (and consequently Th1 function) is regulated, we have determined the gene structure and investigated the mechanisms of transcriptional control and cell type expression. The mouse IL-18 gene comprises seven exons distributed over 26 kb. Exons 1 and 2 of this gene are 5'-noncoding exons. Promoter activity was detected upstream of these noncoding exons in two distinct regions. Both promoters are TATA-less and not G+C rich. The promoter activity located upstream of exon 2 was shown to act constitutively, while the activity located upstream of exon 1 was up-regulated in activated macrophage and T cell lines. IL-18 gene expression may be regulated in a wide range of cell types by the activities of these two distinct promoters. IL-18 is known to be synthesized as a precursor, pro-IL-18, and its maturation is controlled by IL-1beta-converting enzyme (ICE). We observed concordant expression of IL-18 and ICE mRNAs in a wide range of cell types, unlike the more restricted expression of IL-12 p40 mRNA. The widespread IL-18 mRNA distribution and the special relationship with ICE lead us to the hypothesis that IL-18 expression may be coupled with apoptotic processes involving activation of ICE or ICE-like proteinase. |
| first_indexed | 2024-03-06T21:38:33Z |
| format | Journal article |
| id | oxford-uuid:47196790-92a6-4bdf-a085-e1f0509598a1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:38:33Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:47196790-92a6-4bdf-a085-e1f0509598a12022-03-26T15:17:57ZRegulation of IL-18 (IFN-gamma-inducing factor) gene expression.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:47196790-92a6-4bdf-a085-e1f0509598a1EnglishSymplectic Elements at Oxford1997Tone, MThompson, SATone, YFairchild, PWaldmann, HIL-18 (also known as IFN-gamma-inducing factor), although structurally unrelated to IL-12, shares with it the role of activating NK cells and polarizing T cells toward Th1 cell function. To understand how the IL-18 gene (and consequently Th1 function) is regulated, we have determined the gene structure and investigated the mechanisms of transcriptional control and cell type expression. The mouse IL-18 gene comprises seven exons distributed over 26 kb. Exons 1 and 2 of this gene are 5'-noncoding exons. Promoter activity was detected upstream of these noncoding exons in two distinct regions. Both promoters are TATA-less and not G+C rich. The promoter activity located upstream of exon 2 was shown to act constitutively, while the activity located upstream of exon 1 was up-regulated in activated macrophage and T cell lines. IL-18 gene expression may be regulated in a wide range of cell types by the activities of these two distinct promoters. IL-18 is known to be synthesized as a precursor, pro-IL-18, and its maturation is controlled by IL-1beta-converting enzyme (ICE). We observed concordant expression of IL-18 and ICE mRNAs in a wide range of cell types, unlike the more restricted expression of IL-12 p40 mRNA. The widespread IL-18 mRNA distribution and the special relationship with ICE lead us to the hypothesis that IL-18 expression may be coupled with apoptotic processes involving activation of ICE or ICE-like proteinase. |
| spellingShingle | Tone, M Thompson, SA Tone, Y Fairchild, P Waldmann, H Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. |
| title | Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. |
| title_full | Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. |
| title_fullStr | Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. |
| title_full_unstemmed | Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. |
| title_short | Regulation of IL-18 (IFN-gamma-inducing factor) gene expression. |
| title_sort | regulation of il 18 ifn gamma inducing factor gene expression |
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