Characterising biomolecular interactions and dynamics with mass photometry
We review recent advances in our ability to characterise biomolecular structure, interactions and associated dynamics by mass photometry (MP), the label-free detection and mass measurement of individual biomolecules in solution. Molecular counting and identification provides direct access to relativ...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2022
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| _version_ | 1797109249873018880 |
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| author | Asor, R Kukura, P |
| author_facet | Asor, R Kukura, P |
| author_sort | Asor, R |
| collection | OXFORD |
| description | We review recent advances in our ability to characterise biomolecular structure, interactions and associated dynamics by mass photometry (MP), the label-free detection and mass measurement of individual biomolecules in solution. Molecular counting and identification provides direct access to relative abundance, and thereby affinities, while associated dynamics yield on- and off-rates. The molecular resolution afforded by MP enables these measurements as a function of stoichiometry and assembly at equilibrium, as opposed to the majority of existing solution-based methods. Together with future improvements in terms of assays and technological performance, MP is likely to provide mechanistic details of complex biomolecular processes. |
| first_indexed | 2024-03-07T07:39:08Z |
| format | Journal article |
| id | oxford-uuid:472ce7dc-1af4-4ff7-9ef9-e09c05bd332c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:39:08Z |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:472ce7dc-1af4-4ff7-9ef9-e09c05bd332c2023-04-14T09:07:15ZCharacterising biomolecular interactions and dynamics with mass photometryJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:472ce7dc-1af4-4ff7-9ef9-e09c05bd332cEnglishSymplectic ElementsElsevier2022Asor, RKukura, PWe review recent advances in our ability to characterise biomolecular structure, interactions and associated dynamics by mass photometry (MP), the label-free detection and mass measurement of individual biomolecules in solution. Molecular counting and identification provides direct access to relative abundance, and thereby affinities, while associated dynamics yield on- and off-rates. The molecular resolution afforded by MP enables these measurements as a function of stoichiometry and assembly at equilibrium, as opposed to the majority of existing solution-based methods. Together with future improvements in terms of assays and technological performance, MP is likely to provide mechanistic details of complex biomolecular processes. |
| spellingShingle | Asor, R Kukura, P Characterising biomolecular interactions and dynamics with mass photometry |
| title | Characterising biomolecular interactions and dynamics with mass photometry |
| title_full | Characterising biomolecular interactions and dynamics with mass photometry |
| title_fullStr | Characterising biomolecular interactions and dynamics with mass photometry |
| title_full_unstemmed | Characterising biomolecular interactions and dynamics with mass photometry |
| title_short | Characterising biomolecular interactions and dynamics with mass photometry |
| title_sort | characterising biomolecular interactions and dynamics with mass photometry |
| work_keys_str_mv | AT asorr characterisingbiomolecularinteractionsanddynamicswithmassphotometry AT kukurap characterisingbiomolecularinteractionsanddynamicswithmassphotometry |