Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde.
(Chemical Equation Presented) Formaldetected: Application of 1H- and 1D 13C HSQC NMR spectroscopy methods for monitoring the activity of a 2-oxoglutarate dependent histone Nε-emethyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, inc...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2010
|
| _version_ | 1797066209231896576 |
|---|---|
| author | Hopkinson, R Hamed, R Rose, N Claridge, T Schofield, C |
| author_facet | Hopkinson, R Hamed, R Rose, N Claridge, T Schofield, C |
| author_sort | Hopkinson, R |
| collection | OXFORD |
| description | (Chemical Equation Presented) Formaldetected: Application of 1H- and 1D 13C HSQC NMR spectroscopy methods for monitoring the activity of a 2-oxoglutarate dependent histone Nε-emethyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, including the direct observation of hydrated formaldehyde resulting from enzyme-catalysed demethylation. © 2010 Wiley-VCH Verlag GmbHand Co. KGaA. |
| first_indexed | 2024-03-06T21:39:09Z |
| format | Journal article |
| id | oxford-uuid:474ceeb1-6fb7-413d-8f76-a3fce0ff0283 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:39:09Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:474ceeb1-6fb7-413d-8f76-a3fce0ff02832022-03-26T15:19:21ZMonitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:474ceeb1-6fb7-413d-8f76-a3fce0ff0283EnglishSymplectic Elements at Oxford2010Hopkinson, RHamed, RRose, NClaridge, TSchofield, C(Chemical Equation Presented) Formaldetected: Application of 1H- and 1D 13C HSQC NMR spectroscopy methods for monitoring the activity of a 2-oxoglutarate dependent histone Nε-emethyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, including the direct observation of hydrated formaldehyde resulting from enzyme-catalysed demethylation. © 2010 Wiley-VCH Verlag GmbHand Co. KGaA. |
| spellingShingle | Hopkinson, R Hamed, R Rose, N Claridge, T Schofield, C Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde. |
| title | Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde. |
| title_full | Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde. |
| title_fullStr | Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde. |
| title_full_unstemmed | Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde. |
| title_short | Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: direct observation of formaldehyde. |
| title_sort | monitoring the activity of 2 oxoglutarate dependent histone demethylases by nmr spectroscopy direct observation of formaldehyde |
| work_keys_str_mv | AT hopkinsonr monitoringtheactivityof2oxoglutaratedependenthistonedemethylasesbynmrspectroscopydirectobservationofformaldehyde AT hamedr monitoringtheactivityof2oxoglutaratedependenthistonedemethylasesbynmrspectroscopydirectobservationofformaldehyde AT rosen monitoringtheactivityof2oxoglutaratedependenthistonedemethylasesbynmrspectroscopydirectobservationofformaldehyde AT claridget monitoringtheactivityof2oxoglutaratedependenthistonedemethylasesbynmrspectroscopydirectobservationofformaldehyde AT schofieldc monitoringtheactivityof2oxoglutaratedependenthistonedemethylasesbynmrspectroscopydirectobservationofformaldehyde |