Magnetic microbubbles: investigation and design of new formulations for targeted therapy
<p>Targeted therapy is a significant area of research in pharmaceutical and biomedical science. Its overall aim is to achieve maximum impact on malignant cells with minimum side effects to healthy tissue. In this thesis the capabilities of magnetic microbubbles as targeted therapeutic delive...
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| Format: | Thesis |
| Language: | English |
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2014
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| _version_ | 1797066213825708032 |
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| author | Owen, J Joshua Owen |
| author2 | Stride, E |
| author_facet | Stride, E Owen, J Joshua Owen |
| author_sort | Owen, J |
| collection | OXFORD |
| description | <p>Targeted therapy is a significant area of research in pharmaceutical and biomedical science. Its overall aim is to achieve maximum impact on malignant cells with minimum side effects to healthy tissue. In this thesis the capabilities of magnetic microbubbles as targeted therapeutic delivery vehicles are explored. New characterisation techniques were developed in order to understand and improve the current magnetic microbubble formulation. Electron microscopy was used to analyse the nanoscale structure of microbubble shells and observe nanoparticles attached to the shell surface. A new flow phantom was developed and the targeting of magnetic microbubbles against flow conditions corresponding to those in the human body was found to be feasible in numerous vessel sizes and flow conditions. Magnetic targeting of microbubbles was also observed in a perfused porcine liver model. Magnetic targeting was then attempted against flowing blood and a decrease in targeting efficiency observed. This was also seen for biochemical targeting and collisions with red blood cells identified as the most likely cause. Importantly, the number of magnetically targeted microbubbles significantly exceeded those targeted via biochemical interactions in both blood and water. In the second part of the thesis new types of magnetic microbubble were developed. The first exploits the fusion of nano-scale magnetic droplets with phospholipid microbubbles. In the second magnetic nanoparticles were incorporated directly into the lipid shell. The new magnetic microbubble formulation could be magnetically targeted, observed via contrast ultrasound and was successfully used to deliver siRNA to neuroblastoma cells.</p> |
| first_indexed | 2024-03-06T21:39:13Z |
| format | Thesis |
| id | oxford-uuid:47537fb2-76e2-4e84-94bf-1530c57ff25a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:39:13Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:47537fb2-76e2-4e84-94bf-1530c57ff25a2022-03-26T15:19:28ZMagnetic microbubbles: investigation and design of new formulations for targeted therapyThesishttp://purl.org/coar/resource_type/c_db06uuid:47537fb2-76e2-4e84-94bf-1530c57ff25aBiomedical engineeringEnglishOxford University Research Archive - Valet2014Owen, JJoshua OwenStride, E<p>Targeted therapy is a significant area of research in pharmaceutical and biomedical science. Its overall aim is to achieve maximum impact on malignant cells with minimum side effects to healthy tissue. In this thesis the capabilities of magnetic microbubbles as targeted therapeutic delivery vehicles are explored. New characterisation techniques were developed in order to understand and improve the current magnetic microbubble formulation. Electron microscopy was used to analyse the nanoscale structure of microbubble shells and observe nanoparticles attached to the shell surface. A new flow phantom was developed and the targeting of magnetic microbubbles against flow conditions corresponding to those in the human body was found to be feasible in numerous vessel sizes and flow conditions. Magnetic targeting of microbubbles was also observed in a perfused porcine liver model. Magnetic targeting was then attempted against flowing blood and a decrease in targeting efficiency observed. This was also seen for biochemical targeting and collisions with red blood cells identified as the most likely cause. Importantly, the number of magnetically targeted microbubbles significantly exceeded those targeted via biochemical interactions in both blood and water. In the second part of the thesis new types of magnetic microbubble were developed. The first exploits the fusion of nano-scale magnetic droplets with phospholipid microbubbles. In the second magnetic nanoparticles were incorporated directly into the lipid shell. The new magnetic microbubble formulation could be magnetically targeted, observed via contrast ultrasound and was successfully used to deliver siRNA to neuroblastoma cells.</p> |
| spellingShingle | Biomedical engineering Owen, J Joshua Owen Magnetic microbubbles: investigation and design of new formulations for targeted therapy |
| title | Magnetic microbubbles: investigation and design of new formulations for targeted therapy |
| title_full | Magnetic microbubbles: investigation and design of new formulations for targeted therapy |
| title_fullStr | Magnetic microbubbles: investigation and design of new formulations for targeted therapy |
| title_full_unstemmed | Magnetic microbubbles: investigation and design of new formulations for targeted therapy |
| title_short | Magnetic microbubbles: investigation and design of new formulations for targeted therapy |
| title_sort | magnetic microbubbles investigation and design of new formulations for targeted therapy |
| topic | Biomedical engineering |
| work_keys_str_mv | AT owenj magneticmicrobubblesinvestigationanddesignofnewformulationsfortargetedtherapy AT joshuaowen magneticmicrobubblesinvestigationanddesignofnewformulationsfortargetedtherapy |