Stimulation of osteoclast formation by inflammatory synovial fluid
Peri-articular bone resorption is a feature of arthritis due to crystal deposition and rheumatoid disease. Under these conditions, the synovial fluid contains numerous inflammatory cells that produced cytokines and growth factors which promote osteoclast formation. The aim of the study was to determ...
| Glavni autori: | , , , , , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
Springer
2006
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| Teme: |
| _version_ | 1826270359825940480 |
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| author | Adamopoulos, I Danks, L Itonaga, I Locklin, R Sabokbar, A Ferguson, D Athanasou, N |
| author2 | European Society of Pathology |
| author_facet | European Society of Pathology Adamopoulos, I Danks, L Itonaga, I Locklin, R Sabokbar, A Ferguson, D Athanasou, N |
| author_sort | Adamopoulos, I |
| collection | OXFORD |
| description | Peri-articular bone resorption is a feature of arthritis due to crystal deposition and rheumatoid disease. Under these conditions, the synovial fluid contains numerous inflammatory cells that produced cytokines and growth factors which promote osteoclast formation. The aim of the study was to determine whether inflammatory synovial fluid stimulates the formation of osteoclasts. Synovial fluid from rheumatoid arthritis (RA), pyrophosphate arthropathy (PPA) and osteoarthritis (OA) patients was added to cultures (n=8) of human peripheral blood mononuclear cells (PBMCs) in the presence and absence of macrophage colony-stimulating factor (M-CSF) and the receptor activator of NF-KB ligand (RANKL). Osteoclast formation was assessed by the formation of cells positive for tartrare-resistant and phosphatase (TRAP) and vitronectin receptor (VNR) and the extent of lacunar resorption. The addition of 10% OA, RA and PPA synovial fluid to PBMC cultures resulted in the formation of numerous multinicleated or mononuclear TRAP⁺ and VNR⁺ cells which were capable of lacunar resorption. In contrast to PBMC cultures incubated with OA synovial fluid, there was marked stimulation of osteoclast formation and resorption in cultures containing inflammatory RA and PPA synovial fluid which contained high levels of tumour necrosis factor alpha, a factor which is known to stimulate RANKL-induced osteoclast formation. |
| first_indexed | 2024-03-06T21:39:35Z |
| format | Journal article |
| id | oxford-uuid:4772a439-5bea-4148-8e9d-eded8caed0f1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:39:35Z |
| publishDate | 2006 |
| publisher | Springer |
| record_format | dspace |
| spelling | oxford-uuid:4772a439-5bea-4148-8e9d-eded8caed0f12022-03-26T15:20:13ZStimulation of osteoclast formation by inflammatory synovial fluidJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4772a439-5bea-4148-8e9d-eded8caed0f1OrthopaedicsEnglishOxford University Research Archive - ValetSpringer2006Adamopoulos, IDanks, LItonaga, ILocklin, RSabokbar, AFerguson, DAthanasou, NEuropean Society of PathologyPeri-articular bone resorption is a feature of arthritis due to crystal deposition and rheumatoid disease. Under these conditions, the synovial fluid contains numerous inflammatory cells that produced cytokines and growth factors which promote osteoclast formation. The aim of the study was to determine whether inflammatory synovial fluid stimulates the formation of osteoclasts. Synovial fluid from rheumatoid arthritis (RA), pyrophosphate arthropathy (PPA) and osteoarthritis (OA) patients was added to cultures (n=8) of human peripheral blood mononuclear cells (PBMCs) in the presence and absence of macrophage colony-stimulating factor (M-CSF) and the receptor activator of NF-KB ligand (RANKL). Osteoclast formation was assessed by the formation of cells positive for tartrare-resistant and phosphatase (TRAP) and vitronectin receptor (VNR) and the extent of lacunar resorption. The addition of 10% OA, RA and PPA synovial fluid to PBMC cultures resulted in the formation of numerous multinicleated or mononuclear TRAP⁺ and VNR⁺ cells which were capable of lacunar resorption. In contrast to PBMC cultures incubated with OA synovial fluid, there was marked stimulation of osteoclast formation and resorption in cultures containing inflammatory RA and PPA synovial fluid which contained high levels of tumour necrosis factor alpha, a factor which is known to stimulate RANKL-induced osteoclast formation. |
| spellingShingle | Orthopaedics Adamopoulos, I Danks, L Itonaga, I Locklin, R Sabokbar, A Ferguson, D Athanasou, N Stimulation of osteoclast formation by inflammatory synovial fluid |
| title | Stimulation of osteoclast formation by inflammatory synovial fluid |
| title_full | Stimulation of osteoclast formation by inflammatory synovial fluid |
| title_fullStr | Stimulation of osteoclast formation by inflammatory synovial fluid |
| title_full_unstemmed | Stimulation of osteoclast formation by inflammatory synovial fluid |
| title_short | Stimulation of osteoclast formation by inflammatory synovial fluid |
| title_sort | stimulation of osteoclast formation by inflammatory synovial fluid |
| topic | Orthopaedics |
| work_keys_str_mv | AT adamopoulosi stimulationofosteoclastformationbyinflammatorysynovialfluid AT danksl stimulationofosteoclastformationbyinflammatorysynovialfluid AT itonagai stimulationofosteoclastformationbyinflammatorysynovialfluid AT locklinr stimulationofosteoclastformationbyinflammatorysynovialfluid AT sabokbara stimulationofosteoclastformationbyinflammatorysynovialfluid AT fergusond stimulationofosteoclastformationbyinflammatorysynovialfluid AT athanasoun stimulationofosteoclastformationbyinflammatorysynovialfluid |