On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
Species restrictions in immune cell interactions have been demonstrated both in Ag-specific responses of T lymphocytes and the phenomenon of natural attachment. To determine the possible contribution of adhesion receptors to these restrictions, we have studied binding between the murine and human ho...
| Egile Nagusiak: | , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
1990
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| _version_ | 1826270362092961792 |
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| author | Johnston, S Dustin, M Hibbs, M Springer, T |
| author_facet | Johnston, S Dustin, M Hibbs, M Springer, T |
| author_sort | Johnston, S |
| collection | OXFORD |
| description | Species restrictions in immune cell interactions have been demonstrated both in Ag-specific responses of T lymphocytes and the phenomenon of natural attachment. To determine the possible contribution of adhesion receptors to these restrictions, we have studied binding between the murine and human homologues of LFA-1 (CD11a/CD18) and ICAM employing purified human LFA-1 and ICAM-1 (CD54) bound to solid substrates. Murine cell lines bind to purified human LFA-1 through ICAM-1 and at least one other counter-receptor. This provides evidence for multiple counter-receptors for LFA-1 in the mouse as well as in the human. In contrast to binding of murine ICAM-1 to human LFA-1, murine LFA-1 does not bind to human ICAM-1. The species specificity maps to the LFA-1 alpha subunit, because mouse x human hybrid cells expressing the human alpha subunit associated with a mouse beta subunit bind to human ICAM-1, whereas those with a human beta subunit associated with a murine alpha subunit do not. Increased adhesiveness for ICAM-1 stimulated by phorbol esters could be demonstrated for hybrid LFA-1 molecules with human alpha and murine beta subunits. |
| first_indexed | 2024-03-06T21:39:38Z |
| format | Journal article |
| id | oxford-uuid:4777e3a6-16fb-45c2-9844-e2c5065d8ad5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:39:38Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:4777e3a6-16fb-45c2-9844-e2c5065d8ad52022-03-26T15:20:20ZOn the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4777e3a6-16fb-45c2-9844-e2c5065d8ad5EnglishSymplectic Elements at Oxford1990Johnston, SDustin, MHibbs, MSpringer, TSpecies restrictions in immune cell interactions have been demonstrated both in Ag-specific responses of T lymphocytes and the phenomenon of natural attachment. To determine the possible contribution of adhesion receptors to these restrictions, we have studied binding between the murine and human homologues of LFA-1 (CD11a/CD18) and ICAM employing purified human LFA-1 and ICAM-1 (CD54) bound to solid substrates. Murine cell lines bind to purified human LFA-1 through ICAM-1 and at least one other counter-receptor. This provides evidence for multiple counter-receptors for LFA-1 in the mouse as well as in the human. In contrast to binding of murine ICAM-1 to human LFA-1, murine LFA-1 does not bind to human ICAM-1. The species specificity maps to the LFA-1 alpha subunit, because mouse x human hybrid cells expressing the human alpha subunit associated with a mouse beta subunit bind to human ICAM-1, whereas those with a human beta subunit associated with a murine alpha subunit do not. Increased adhesiveness for ICAM-1 stimulated by phorbol esters could be demonstrated for hybrid LFA-1 molecules with human alpha and murine beta subunits. |
| spellingShingle | Johnston, S Dustin, M Hibbs, M Springer, T On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules. |
| title | On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules. |
| title_full | On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules. |
| title_fullStr | On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules. |
| title_full_unstemmed | On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules. |
| title_short | On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules. |
| title_sort | on the species specificity of the interaction of lfa 1 with intercellular adhesion molecules |
| work_keys_str_mv | AT johnstons onthespeciesspecificityoftheinteractionoflfa1withintercellularadhesionmolecules AT dustinm onthespeciesspecificityoftheinteractionoflfa1withintercellularadhesionmolecules AT hibbsm onthespeciesspecificityoftheinteractionoflfa1withintercellularadhesionmolecules AT springert onthespeciesspecificityoftheinteractionoflfa1withintercellularadhesionmolecules |