Effects of SV40 transformation on intercellular gap junctional communication in human fibroblasts.
The effect of SV40 viral transformation of human fibroblasts on intercellular gap junctional communication (IJC) was investigated using a short-term quantitative assay. IJC was measured using metabolic cooperation in a coculture system of argininosuccinate synthetase- and argininosuccinate lyase-def...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1988
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| _version_ | 1797066264573640704 |
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| author | Rosen, A Van Der Merwe, P Davidson, J |
| author_facet | Rosen, A Van Der Merwe, P Davidson, J |
| author_sort | Rosen, A |
| collection | OXFORD |
| description | The effect of SV40 viral transformation of human fibroblasts on intercellular gap junctional communication (IJC) was investigated using a short-term quantitative assay. IJC was measured using metabolic cooperation in a coculture system of argininosuccinate synthetase- and argininosuccinate lyase-deficient human fibroblasts. These cell lines were transformed with origin-defective adenovirus/SV40 recombinant virions, and IJC was determined both between transformed cells (homologous IJC) and between transformed and untransformed cells (heterologous IJC). At equivalent cell densities, homologous IJC between transformed cells was reduced to 25-55% of the level between untransformed cells. Intermediate levels of IJC (50-70% of normal) were observed in heterologous cocultures of transformed with untransformed cells. Transformed and untransformed cells were equally sensitive to inhibition of IJC by phorbol esters and by glycyrrhetinic acid, and also did not differ in the degree of upregulation of IJC by forskolin. We conclude that SV40 transformation of human fibroblasts leads to a partial impairment of IJC which is additive when both communicating partners are transformed. |
| first_indexed | 2024-03-06T21:39:58Z |
| format | Journal article |
| id | oxford-uuid:4794601e-453f-43ea-b01d-0b1deceedadd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:39:58Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:4794601e-453f-43ea-b01d-0b1deceedadd2022-03-26T15:20:55ZEffects of SV40 transformation on intercellular gap junctional communication in human fibroblasts.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4794601e-453f-43ea-b01d-0b1deceedaddEnglishSymplectic Elements at Oxford1988Rosen, AVan Der Merwe, PDavidson, JThe effect of SV40 viral transformation of human fibroblasts on intercellular gap junctional communication (IJC) was investigated using a short-term quantitative assay. IJC was measured using metabolic cooperation in a coculture system of argininosuccinate synthetase- and argininosuccinate lyase-deficient human fibroblasts. These cell lines were transformed with origin-defective adenovirus/SV40 recombinant virions, and IJC was determined both between transformed cells (homologous IJC) and between transformed and untransformed cells (heterologous IJC). At equivalent cell densities, homologous IJC between transformed cells was reduced to 25-55% of the level between untransformed cells. Intermediate levels of IJC (50-70% of normal) were observed in heterologous cocultures of transformed with untransformed cells. Transformed and untransformed cells were equally sensitive to inhibition of IJC by phorbol esters and by glycyrrhetinic acid, and also did not differ in the degree of upregulation of IJC by forskolin. We conclude that SV40 transformation of human fibroblasts leads to a partial impairment of IJC which is additive when both communicating partners are transformed. |
| spellingShingle | Rosen, A Van Der Merwe, P Davidson, J Effects of SV40 transformation on intercellular gap junctional communication in human fibroblasts. |
| title | Effects of SV40 transformation on intercellular gap junctional communication in human fibroblasts. |
| title_full | Effects of SV40 transformation on intercellular gap junctional communication in human fibroblasts. |
| title_fullStr | Effects of SV40 transformation on intercellular gap junctional communication in human fibroblasts. |
| title_full_unstemmed | Effects of SV40 transformation on intercellular gap junctional communication in human fibroblasts. |
| title_short | Effects of SV40 transformation on intercellular gap junctional communication in human fibroblasts. |
| title_sort | effects of sv40 transformation on intercellular gap junctional communication in human fibroblasts |
| work_keys_str_mv | AT rosena effectsofsv40transformationonintercellulargapjunctionalcommunicationinhumanfibroblasts AT vandermerwep effectsofsv40transformationonintercellulargapjunctionalcommunicationinhumanfibroblasts AT davidsonj effectsofsv40transformationonintercellulargapjunctionalcommunicationinhumanfibroblasts |