Induction of human antigen-specific suppressor factors in vitro.
Based on methods used for the in vitro induction of antigen-specific suppressor cells in the mouse, we have cultured Ficoll-Isopaque-separated human blood cells with high dose of antigen (100 microgram/ml) in Marbrook culture vessels for 4 days. The resulting cells, when further recultured for 24 hr...
| Main Authors: | , , , |
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| Format: | Journal article |
| Jezik: | English |
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1981
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| _version_ | 1826270402414903296 |
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| author | Kontiainen, S Woody, J Rees, A Feldmann, M |
| author_facet | Kontiainen, S Woody, J Rees, A Feldmann, M |
| author_sort | Kontiainen, S |
| collection | OXFORD |
| description | Based on methods used for the in vitro induction of antigen-specific suppressor cells in the mouse, we have cultured Ficoll-Isopaque-separated human blood cells with high dose of antigen (100 microgram/ml) in Marbrook culture vessels for 4 days. The resulting cells, when further recultured for 24 hr with a low dose of antigen (1 microgram/ml), released into the supernatant material, termed 'suppressor factor', which inhibited, in an antigen-specific manner, the antibody response of mouse spleen cells in culture. The suppressor factor was analysed using immunoabsorbents, and was bound to and eluted from specific antigen, concanavalin A and lentil lectin, anti-human Ia antibodies, and anti-mouse suppressor factor antibodies, but was not bound to antibodies against human IgG. |
| first_indexed | 2024-03-06T21:40:15Z |
| format | Journal article |
| id | oxford-uuid:47aed313-7e32-4892-bba5-e9e57794ce95 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:40:15Z |
| publishDate | 1981 |
| record_format | dspace |
| spelling | oxford-uuid:47aed313-7e32-4892-bba5-e9e57794ce952022-03-26T15:21:25ZInduction of human antigen-specific suppressor factors in vitro.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:47aed313-7e32-4892-bba5-e9e57794ce95EnglishSymplectic Elements at Oxford1981Kontiainen, SWoody, JRees, AFeldmann, MBased on methods used for the in vitro induction of antigen-specific suppressor cells in the mouse, we have cultured Ficoll-Isopaque-separated human blood cells with high dose of antigen (100 microgram/ml) in Marbrook culture vessels for 4 days. The resulting cells, when further recultured for 24 hr with a low dose of antigen (1 microgram/ml), released into the supernatant material, termed 'suppressor factor', which inhibited, in an antigen-specific manner, the antibody response of mouse spleen cells in culture. The suppressor factor was analysed using immunoabsorbents, and was bound to and eluted from specific antigen, concanavalin A and lentil lectin, anti-human Ia antibodies, and anti-mouse suppressor factor antibodies, but was not bound to antibodies against human IgG. |
| spellingShingle | Kontiainen, S Woody, J Rees, A Feldmann, M Induction of human antigen-specific suppressor factors in vitro. |
| title | Induction of human antigen-specific suppressor factors in vitro. |
| title_full | Induction of human antigen-specific suppressor factors in vitro. |
| title_fullStr | Induction of human antigen-specific suppressor factors in vitro. |
| title_full_unstemmed | Induction of human antigen-specific suppressor factors in vitro. |
| title_short | Induction of human antigen-specific suppressor factors in vitro. |
| title_sort | induction of human antigen specific suppressor factors in vitro |
| work_keys_str_mv | AT kontiainens inductionofhumanantigenspecificsuppressorfactorsinvitro AT woodyj inductionofhumanantigenspecificsuppressorfactorsinvitro AT reesa inductionofhumanantigenspecificsuppressorfactorsinvitro AT feldmannm inductionofhumanantigenspecificsuppressorfactorsinvitro |