Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime.
Human immunodeficiency virus (HIV) vaccine candidates were previously constructed as a string of cytotoxic T lymphocyte (CTL) epitopes delivered and expressed using DNA and modified virus Ankara (MVA; an attenuated vaccinia virus) vectors. These vaccines were shown to induce interferon (IFN)-gamma-p...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1998
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| _version_ | 1797066540203376640 |
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| author | Hanke, T Blanchard, T Schneider, J Hannan, C Becker, M Gilbert, S Hill, A Smith, G McMichael, A |
| author_facet | Hanke, T Blanchard, T Schneider, J Hannan, C Becker, M Gilbert, S Hill, A Smith, G McMichael, A |
| author_sort | Hanke, T |
| collection | OXFORD |
| description | Human immunodeficiency virus (HIV) vaccine candidates were previously constructed as a string of cytotoxic T lymphocyte (CTL) epitopes delivered and expressed using DNA and modified virus Ankara (MVA; an attenuated vaccinia virus) vectors. These vaccines were shown to induce interferon (IFN)-gamma-producing and cytolytic CD8+ T cells after a single vaccine administration. In the course of this work, immunization protocols were sought which would improve the levels of induced HIV-specific T cells. It was found that previous immunological exposure to MVA reduced the efficiency of subsequent priming and boosting using the same vaccine vehicle. However, a combined regime whereby the animals were first primed with the DNA vaccine and then boosted with MVA was the most potent protocol for the induction of both interferon-gamma-producing and cytolytic T cells against two CTL epitopes simultaneously. The general applicability of this novel vaccination method for induction of major histocompatibility complex class I-restricted T cells is discussed. |
| first_indexed | 2024-03-06T21:43:31Z |
| format | Journal article |
| id | oxford-uuid:48c316e6-dcab-4a06-a645-e47804a358ab |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:43:31Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:48c316e6-dcab-4a06-a645-e47804a358ab2022-03-26T15:27:33ZEnhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:48c316e6-dcab-4a06-a645-e47804a358abEnglishSymplectic Elements at Oxford1998Hanke, TBlanchard, TSchneider, JHannan, CBecker, MGilbert, SHill, ASmith, GMcMichael, AHuman immunodeficiency virus (HIV) vaccine candidates were previously constructed as a string of cytotoxic T lymphocyte (CTL) epitopes delivered and expressed using DNA and modified virus Ankara (MVA; an attenuated vaccinia virus) vectors. These vaccines were shown to induce interferon (IFN)-gamma-producing and cytolytic CD8+ T cells after a single vaccine administration. In the course of this work, immunization protocols were sought which would improve the levels of induced HIV-specific T cells. It was found that previous immunological exposure to MVA reduced the efficiency of subsequent priming and boosting using the same vaccine vehicle. However, a combined regime whereby the animals were first primed with the DNA vaccine and then boosted with MVA was the most potent protocol for the induction of both interferon-gamma-producing and cytolytic T cells against two CTL epitopes simultaneously. The general applicability of this novel vaccination method for induction of major histocompatibility complex class I-restricted T cells is discussed. |
| spellingShingle | Hanke, T Blanchard, T Schneider, J Hannan, C Becker, M Gilbert, S Hill, A Smith, G McMichael, A Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime. |
| title | Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime. |
| title_full | Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime. |
| title_fullStr | Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime. |
| title_full_unstemmed | Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime. |
| title_short | Enhancement of MHC class I-restricted peptide-specific T cell induction by a DNA prime/MVA boost vaccination regime. |
| title_sort | enhancement of mhc class i restricted peptide specific t cell induction by a dna prime mva boost vaccination regime |
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