Elucidation of drug resistant mutations of Mycobacterium tuberculosis by whole genome sequencing from North India
INTRODUCTION:Rapid diagnosis of drug resistant tuberculosis is required for better patient management and treatment outcome. Whole-genome sequencing (WGS) can detect single nucleotide polymorphisms (SNPs) and deletions/insertions which are responsible for mostMycobacterium tuberculosis drug resistan...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2019
|
| _version_ | 1797066848208945152 |
|---|---|
| author | Sethi, S Hao, Y Brown, S Walker, T Yadav, R Zaman, K Aggarwal, A Behera, D |
| author_facet | Sethi, S Hao, Y Brown, S Walker, T Yadav, R Zaman, K Aggarwal, A Behera, D |
| author_sort | Sethi, S |
| collection | OXFORD |
| description | INTRODUCTION:Rapid diagnosis of drug resistant tuberculosis is required for better patient management and treatment outcome. Whole-genome sequencing (WGS) can detect single nucleotide polymorphisms (SNPs) and deletions/insertions which are responsible for mostMycobacterium tuberculosis drug resistance. WGS is being performed at scale in high-income countries but there are still limited reports of its use in India. METHOD:In this study, 33 clinicalM. tuberculosis isolates were taken from Mycobacterial repository in Chandigarh and were whole-genome sequenced. Phenotypic drug susceptibility testing was performed according to WHO recommendations. Four were considered culture contaminated. RESULTS:Among the other 29 isolates, 21(72.4%) were multi-drug resistance (MDR-TB) and one was extensively-drug resistant (XDR-TB). The most common mutations observed for isoniazid, rifampicin, ofloxacin and kanamycin werekatG_S315 T, rpoB_S450 L, gyrA_A90 V and rrs_A1401 G respectively. The isolates belonged to lineage 2 and 3, with most MDR-TB among lineage 2 isolates. CONCLUSION:Whole-Genome Sequencing ofMycobacterium tuberculosis offers the detection of drug resistance to all drugs in a single test and also provides insight into the evolution and drug-resistant tuberculosis. |
| first_indexed | 2024-03-06T21:47:53Z |
| format | Journal article |
| id | oxford-uuid:4a3af22d-810c-4bfd-b80d-17ca2c694438 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:47:53Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:4a3af22d-810c-4bfd-b80d-17ca2c6944382022-03-26T15:36:19ZElucidation of drug resistant mutations of Mycobacterium tuberculosis by whole genome sequencing from North IndiaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4a3af22d-810c-4bfd-b80d-17ca2c694438EnglishSymplectic Elements at OxfordElsevier2019Sethi, SHao, YBrown, SWalker, TYadav, RZaman, KAggarwal, ABehera, DINTRODUCTION:Rapid diagnosis of drug resistant tuberculosis is required for better patient management and treatment outcome. Whole-genome sequencing (WGS) can detect single nucleotide polymorphisms (SNPs) and deletions/insertions which are responsible for mostMycobacterium tuberculosis drug resistance. WGS is being performed at scale in high-income countries but there are still limited reports of its use in India. METHOD:In this study, 33 clinicalM. tuberculosis isolates were taken from Mycobacterial repository in Chandigarh and were whole-genome sequenced. Phenotypic drug susceptibility testing was performed according to WHO recommendations. Four were considered culture contaminated. RESULTS:Among the other 29 isolates, 21(72.4%) were multi-drug resistance (MDR-TB) and one was extensively-drug resistant (XDR-TB). The most common mutations observed for isoniazid, rifampicin, ofloxacin and kanamycin werekatG_S315 T, rpoB_S450 L, gyrA_A90 V and rrs_A1401 G respectively. The isolates belonged to lineage 2 and 3, with most MDR-TB among lineage 2 isolates. CONCLUSION:Whole-Genome Sequencing ofMycobacterium tuberculosis offers the detection of drug resistance to all drugs in a single test and also provides insight into the evolution and drug-resistant tuberculosis. |
| spellingShingle | Sethi, S Hao, Y Brown, S Walker, T Yadav, R Zaman, K Aggarwal, A Behera, D Elucidation of drug resistant mutations of Mycobacterium tuberculosis by whole genome sequencing from North India |
| title | Elucidation of drug resistant mutations of Mycobacterium tuberculosis by whole genome sequencing from North India |
| title_full | Elucidation of drug resistant mutations of Mycobacterium tuberculosis by whole genome sequencing from North India |
| title_fullStr | Elucidation of drug resistant mutations of Mycobacterium tuberculosis by whole genome sequencing from North India |
| title_full_unstemmed | Elucidation of drug resistant mutations of Mycobacterium tuberculosis by whole genome sequencing from North India |
| title_short | Elucidation of drug resistant mutations of Mycobacterium tuberculosis by whole genome sequencing from North India |
| title_sort | elucidation of drug resistant mutations of mycobacterium tuberculosis by whole genome sequencing from north india |
| work_keys_str_mv | AT sethis elucidationofdrugresistantmutationsofmycobacteriumtuberculosisbywholegenomesequencingfromnorthindia AT haoy elucidationofdrugresistantmutationsofmycobacteriumtuberculosisbywholegenomesequencingfromnorthindia AT browns elucidationofdrugresistantmutationsofmycobacteriumtuberculosisbywholegenomesequencingfromnorthindia AT walkert elucidationofdrugresistantmutationsofmycobacteriumtuberculosisbywholegenomesequencingfromnorthindia AT yadavr elucidationofdrugresistantmutationsofmycobacteriumtuberculosisbywholegenomesequencingfromnorthindia AT zamank elucidationofdrugresistantmutationsofmycobacteriumtuberculosisbywholegenomesequencingfromnorthindia AT aggarwala elucidationofdrugresistantmutationsofmycobacteriumtuberculosisbywholegenomesequencingfromnorthindia AT beherad elucidationofdrugresistantmutationsofmycobacteriumtuberculosisbywholegenomesequencingfromnorthindia |