T cell clones from a Sjogren's syndrome salivary gland biopsy produce high levels of IL-10.
Sjögren's syndrome (SS) is characterized by a focal periductal salivary gland infiltrate consisting mainly of T and B lymphocytes. Most of the T cells bear the memory CD4+ Th1-like phenotype and express high levels of class II, though CD8+ cells are also present. We have studied 17 labial saliv...
| Príomhchruthaitheoirí: | , , , , , , |
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| Formáid: | Journal article |
| Teanga: | English |
| Foilsithe / Cruthaithe: |
1996
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| _version_ | 1826270978530869248 |
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| author | Brookes, S Cohen, S Price, E Webb, L Feldmann, M Maini, R Venables, P |
| author_facet | Brookes, S Cohen, S Price, E Webb, L Feldmann, M Maini, R Venables, P |
| author_sort | Brookes, S |
| collection | OXFORD |
| description | Sjögren's syndrome (SS) is characterized by a focal periductal salivary gland infiltrate consisting mainly of T and B lymphocytes. Most of the T cells bear the memory CD4+ Th1-like phenotype and express high levels of class II, though CD8+ cells are also present. We have studied 17 labial salivary gland and 15 peripheral blood T cell clones from a patient with primary SS. The tissue clones were 71% CD8+ and 29% CD4+, and the peripheral blood-derived clones were 60% CD8+ and 40% CD4+. The CD4+ T cell clones from both the salivary gland and autologous peripheral blood were of the Th1 phenotype, in that they produced interferon-gamma (IFN-gamma) and IL-2 but very little IL-4 after 24 h stimulation with phorbol myristate acetate and anti-CD3 antibody. The salivary gland-derived CD4+ clones produced 15 times more IL-10 (7.92 ng/ml) than peripheral blood-derived CD4+ clones (0.52 ng/ml, P < or = 0.02). The tissue CD8+ clones produced 1.2 times (P < 0.04) more IFN-gamma and CD4+ clones produced 3.5 times less IL-2 (P < 0.02) than the respective PBM-derived clones. The accumulation of Th1-type cells producing high levels of IL-10 in the salivary gland suggests a specific immunoregulatory function at the site of inflammation in SS. |
| first_indexed | 2024-03-06T21:49:23Z |
| format | Journal article |
| id | oxford-uuid:4ab97657-cfe1-4fe1-a121-4f55236bc600 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:49:23Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:4ab97657-cfe1-4fe1-a121-4f55236bc6002022-03-26T15:39:15ZT cell clones from a Sjogren's syndrome salivary gland biopsy produce high levels of IL-10.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4ab97657-cfe1-4fe1-a121-4f55236bc600EnglishSymplectic Elements at Oxford1996Brookes, SCohen, SPrice, EWebb, LFeldmann, MMaini, RVenables, PSjögren's syndrome (SS) is characterized by a focal periductal salivary gland infiltrate consisting mainly of T and B lymphocytes. Most of the T cells bear the memory CD4+ Th1-like phenotype and express high levels of class II, though CD8+ cells are also present. We have studied 17 labial salivary gland and 15 peripheral blood T cell clones from a patient with primary SS. The tissue clones were 71% CD8+ and 29% CD4+, and the peripheral blood-derived clones were 60% CD8+ and 40% CD4+. The CD4+ T cell clones from both the salivary gland and autologous peripheral blood were of the Th1 phenotype, in that they produced interferon-gamma (IFN-gamma) and IL-2 but very little IL-4 after 24 h stimulation with phorbol myristate acetate and anti-CD3 antibody. The salivary gland-derived CD4+ clones produced 15 times more IL-10 (7.92 ng/ml) than peripheral blood-derived CD4+ clones (0.52 ng/ml, P < or = 0.02). The tissue CD8+ clones produced 1.2 times (P < 0.04) more IFN-gamma and CD4+ clones produced 3.5 times less IL-2 (P < 0.02) than the respective PBM-derived clones. The accumulation of Th1-type cells producing high levels of IL-10 in the salivary gland suggests a specific immunoregulatory function at the site of inflammation in SS. |
| spellingShingle | Brookes, S Cohen, S Price, E Webb, L Feldmann, M Maini, R Venables, P T cell clones from a Sjogren's syndrome salivary gland biopsy produce high levels of IL-10. |
| title | T cell clones from a Sjogren's syndrome salivary gland biopsy produce high levels of IL-10. |
| title_full | T cell clones from a Sjogren's syndrome salivary gland biopsy produce high levels of IL-10. |
| title_fullStr | T cell clones from a Sjogren's syndrome salivary gland biopsy produce high levels of IL-10. |
| title_full_unstemmed | T cell clones from a Sjogren's syndrome salivary gland biopsy produce high levels of IL-10. |
| title_short | T cell clones from a Sjogren's syndrome salivary gland biopsy produce high levels of IL-10. |
| title_sort | t cell clones from a sjogren s syndrome salivary gland biopsy produce high levels of il 10 |
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