Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection.
Previous studies on CTL responses in HIV-exposed uninfected individuals assumed that the patients were exposed to replicating HIV, but the possibility that the immune responses detected were primed by exposure to a defective virus or viral antigen could not be excluded. Epidemiological and laborator...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1826270987344150528 |
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| author | Missale, G Papagno, L Penna, A Pilli, M Zerbini, A Vitali, P Pieroni, G Urbani, S Uggeri, J Pinheiro, S Rowland-Jones, S Ferrari, C |
| author_facet | Missale, G Papagno, L Penna, A Pilli, M Zerbini, A Vitali, P Pieroni, G Urbani, S Uggeri, J Pinheiro, S Rowland-Jones, S Ferrari, C |
| author_sort | Missale, G |
| collection | OXFORD |
| description | Previous studies on CTL responses in HIV-exposed uninfected individuals assumed that the patients were exposed to replicating HIV, but the possibility that the immune responses detected were primed by exposure to a defective virus or viral antigen could not be excluded. Epidemiological and laboratory analysis of a nosocomial outbreak of acute hepatitis B unequivocally allowed the identification of an HIV-1- and HBV-co-infected patient with high plasma levels of both viruses, as the source case of the epidemics. This clinical setting provided a natural model for testing the HIV-specific T cell response in patients exposed to blood from a patient with highly replicating HIV. Parenteral exposure to both viruses led to acute hepatitis B in five subjects without evidence of HIV-1 infection. Cryopreserved lymphocytes derived from three exposed patients were tested ex vivo in an ELISPOT assay for IFN-gamma release upon stimulation with peptides from structural and non-structural HIV proteins; one of the patients was also tested with four HLA/class I tetramers. Circulating HIV-specific CD8 cells were detected by tetramer staining and a high frequency of T cells were able to release IFN-gamma upon stimulation with HIV peptides, showing in vivo T cell priming by HIV. These results unequivocally demonstrate a HIV-specific cell-mediated immune response in the absence of infection after exposure to highly replicating HIV. |
| first_indexed | 2024-03-06T21:49:30Z |
| format | Journal article |
| id | oxford-uuid:4ac204d8-a250-4023-9339-1ceb5ce4d198 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:49:30Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:4ac204d8-a250-4023-9339-1ceb5ce4d1982022-03-26T15:39:33ZParenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4ac204d8-a250-4023-9339-1ceb5ce4d198EnglishSymplectic Elements at Oxford2004Missale, GPapagno, LPenna, APilli, MZerbini, AVitali, PPieroni, GUrbani, SUggeri, JPinheiro, SRowland-Jones, SFerrari, CPrevious studies on CTL responses in HIV-exposed uninfected individuals assumed that the patients were exposed to replicating HIV, but the possibility that the immune responses detected were primed by exposure to a defective virus or viral antigen could not be excluded. Epidemiological and laboratory analysis of a nosocomial outbreak of acute hepatitis B unequivocally allowed the identification of an HIV-1- and HBV-co-infected patient with high plasma levels of both viruses, as the source case of the epidemics. This clinical setting provided a natural model for testing the HIV-specific T cell response in patients exposed to blood from a patient with highly replicating HIV. Parenteral exposure to both viruses led to acute hepatitis B in five subjects without evidence of HIV-1 infection. Cryopreserved lymphocytes derived from three exposed patients were tested ex vivo in an ELISPOT assay for IFN-gamma release upon stimulation with peptides from structural and non-structural HIV proteins; one of the patients was also tested with four HLA/class I tetramers. Circulating HIV-specific CD8 cells were detected by tetramer staining and a high frequency of T cells were able to release IFN-gamma upon stimulation with HIV peptides, showing in vivo T cell priming by HIV. These results unequivocally demonstrate a HIV-specific cell-mediated immune response in the absence of infection after exposure to highly replicating HIV. |
| spellingShingle | Missale, G Papagno, L Penna, A Pilli, M Zerbini, A Vitali, P Pieroni, G Urbani, S Uggeri, J Pinheiro, S Rowland-Jones, S Ferrari, C Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. |
| title | Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. |
| title_full | Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. |
| title_fullStr | Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. |
| title_full_unstemmed | Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. |
| title_short | Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. |
| title_sort | parenteral exposure to high hiv viremia leads to virus specific t cell priming without evidence of infection |
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