Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: an epidemiological and genomic analysis
<p><strong>Objectives</strong></p><p> To investigate the risk of colonization with ESBL-producing Escherichia coli (ESBL-Ec) in humans in Vietnam associated with non-intensive chicken farming. </p><p><strong>Methods</strong></p><p>...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2019
|
| _version_ | 1797067128183980032 |
|---|---|
| author | Nguyen, V Jamrozy, D Matamoros, S Carrique-Mas, J Ho, H Thai, Q Nguyen, T Wagenaar, J Thwaites, G Parkhill, J Schultsz, C Ngo, H |
| author_facet | Nguyen, V Jamrozy, D Matamoros, S Carrique-Mas, J Ho, H Thai, Q Nguyen, T Wagenaar, J Thwaites, G Parkhill, J Schultsz, C Ngo, H |
| author_sort | Nguyen, V |
| collection | OXFORD |
| description | <p><strong>Objectives</strong></p><p> To investigate the risk of colonization with ESBL-producing Escherichia coli (ESBL-Ec) in humans in Vietnam associated with non-intensive chicken farming. </p><p><strong>Methods</strong></p><p> Faecal samples from 204 randomly selected farmers and their chickens, and from 306 age- and sex-matched community-based individuals who did not raise poultry were collected. Antimicrobial usage in chickens and humans was assessed by medicine cabinet surveys. WGS was employed to obtain a high-resolution genomic comparison between ESBL-Ec isolated from humans and chickens. </p><p><strong>Results</strong></p><p> The adjusted prevalence of ESBL-Ec colonization was 20.0% (95% CI 10.8%–29.1%) and 35.2% (95% CI 30.4%–40.1%) in chicken farms and humans in Vietnam, respectively. Colonization with ESBL-Ec in humans was associated with antimicrobial usage (OR = 2.52, 95% CI = 1.08–5.87) but not with involvement in chicken farming. blaCTX-M-55 was the most common ESBL-encoding gene in strains isolated from chickens (74.4%) compared with blaCTX-M-27 in human strains (47.0%). In 3 of 204 (1.5%) of the farms, identical ESBL genes were detected in ESBL-Ec isolated from farmers and their chickens. Genomic similarity indicating recent sharing of ESBL-Ec between chickens and farmers was found in only one of these farms. </p><p><strong>Conclusion</strong></p><p> The integration of epidemiological and genomic data in this study has demonstrated a limited contribution of non-intensive chicken farming to ESBL-Ec colonization in humans in Vietnam and further emphasizes the importance of reducing antimicrobial usage in both human and animal host reservoirs.</p> |
| first_indexed | 2024-03-06T21:51:54Z |
| format | Journal article |
| id | oxford-uuid:4b90ac7d-ca76-491a-8c9b-97c56ca3b56b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:51:54Z |
| publishDate | 2019 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:4b90ac7d-ca76-491a-8c9b-97c56ca3b56b2022-03-26T15:44:21ZLimited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: an epidemiological and genomic analysisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4b90ac7d-ca76-491a-8c9b-97c56ca3b56bEnglishSymplectic Elements at OxfordOxford University Press2019Nguyen, VJamrozy, DMatamoros, SCarrique-Mas, JHo, HThai, QNguyen, TWagenaar, JThwaites, GParkhill, JSchultsz, CNgo, H<p><strong>Objectives</strong></p><p> To investigate the risk of colonization with ESBL-producing Escherichia coli (ESBL-Ec) in humans in Vietnam associated with non-intensive chicken farming. </p><p><strong>Methods</strong></p><p> Faecal samples from 204 randomly selected farmers and their chickens, and from 306 age- and sex-matched community-based individuals who did not raise poultry were collected. Antimicrobial usage in chickens and humans was assessed by medicine cabinet surveys. WGS was employed to obtain a high-resolution genomic comparison between ESBL-Ec isolated from humans and chickens. </p><p><strong>Results</strong></p><p> The adjusted prevalence of ESBL-Ec colonization was 20.0% (95% CI 10.8%–29.1%) and 35.2% (95% CI 30.4%–40.1%) in chicken farms and humans in Vietnam, respectively. Colonization with ESBL-Ec in humans was associated with antimicrobial usage (OR = 2.52, 95% CI = 1.08–5.87) but not with involvement in chicken farming. blaCTX-M-55 was the most common ESBL-encoding gene in strains isolated from chickens (74.4%) compared with blaCTX-M-27 in human strains (47.0%). In 3 of 204 (1.5%) of the farms, identical ESBL genes were detected in ESBL-Ec isolated from farmers and their chickens. Genomic similarity indicating recent sharing of ESBL-Ec between chickens and farmers was found in only one of these farms. </p><p><strong>Conclusion</strong></p><p> The integration of epidemiological and genomic data in this study has demonstrated a limited contribution of non-intensive chicken farming to ESBL-Ec colonization in humans in Vietnam and further emphasizes the importance of reducing antimicrobial usage in both human and animal host reservoirs.</p> |
| spellingShingle | Nguyen, V Jamrozy, D Matamoros, S Carrique-Mas, J Ho, H Thai, Q Nguyen, T Wagenaar, J Thwaites, G Parkhill, J Schultsz, C Ngo, H Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: an epidemiological and genomic analysis |
| title | Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: an epidemiological and genomic analysis |
| title_full | Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: an epidemiological and genomic analysis |
| title_fullStr | Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: an epidemiological and genomic analysis |
| title_full_unstemmed | Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: an epidemiological and genomic analysis |
| title_short | Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam: an epidemiological and genomic analysis |
| title_sort | limited contribution of non intensive chicken farming to esbl producing escherichia coli colonization in humans in vietnam an epidemiological and genomic analysis |
| work_keys_str_mv | AT nguyenv limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT jamrozyd limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT matamoross limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT carriquemasj limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT hoh limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT thaiq limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT nguyent limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT wagenaarj limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT thwaitesg limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT parkhillj limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT schultszc limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis AT ngoh limitedcontributionofnonintensivechickenfarmingtoesblproducingescherichiacolicolonizationinhumansinvietnamanepidemiologicalandgenomicanalysis |