A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics.
We present a peptide library and data resource of >100,000 synthetic, unmodified peptides and their phosphorylated counterparts with known sequences and phosphorylation sites. Analysis of the library by mass spectrometry yielded a data set that we used to evaluate the merits of different sear...
Main Authors: | , , , , , , , , |
---|---|
Format: | Journal article |
Language: | English |
Published: |
2013
|
_version_ | 1797067193851052032 |
---|---|
author | Marx, H Lemeer, S Schliep, J Matheron, L Mohammed, S Cox, J Mann, M Heck, A Kuster, B |
author_facet | Marx, H Lemeer, S Schliep, J Matheron, L Mohammed, S Cox, J Mann, M Heck, A Kuster, B |
author_sort | Marx, H |
collection | OXFORD |
description | We present a peptide library and data resource of >100,000 synthetic, unmodified peptides and their phosphorylated counterparts with known sequences and phosphorylation sites. Analysis of the library by mass spectrometry yielded a data set that we used to evaluate the merits of different search engines (Mascot and Andromeda) and fragmentation methods (beam-type collision-induced dissociation (HCD) and electron transfer dissociation (ETD)) for peptide identification. We also compared the sensitivities and accuracies of phosphorylation-site localization tools (Mascot Delta Score, PTM score and phosphoRS), and we characterized the chromatographic behavior of peptides in the library. We found that HCD identified more peptides and phosphopeptides than did ETD, that phosphopeptides generally eluted later from reversed-phase columns and were easier to identify than unmodified peptides and that current computational tools for proteomics can still be substantially improved. These peptides and spectra will facilitate the development, evaluation and improvement of experimental and computational proteomic strategies, such as separation techniques and the prediction of retention times and fragmentation patterns. |
first_indexed | 2024-03-06T21:52:52Z |
format | Journal article |
id | oxford-uuid:4be83133-1b8b-4364-a2b7-599aa088b5c1 |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-06T21:52:52Z |
publishDate | 2013 |
record_format | dspace |
spelling | oxford-uuid:4be83133-1b8b-4364-a2b7-599aa088b5c12022-03-26T15:46:28ZA large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4be83133-1b8b-4364-a2b7-599aa088b5c1EnglishSymplectic Elements at Oxford2013Marx, HLemeer, SSchliep, JMatheron, LMohammed, SCox, JMann, MHeck, AKuster, BWe present a peptide library and data resource of >100,000 synthetic, unmodified peptides and their phosphorylated counterparts with known sequences and phosphorylation sites. Analysis of the library by mass spectrometry yielded a data set that we used to evaluate the merits of different search engines (Mascot and Andromeda) and fragmentation methods (beam-type collision-induced dissociation (HCD) and electron transfer dissociation (ETD)) for peptide identification. We also compared the sensitivities and accuracies of phosphorylation-site localization tools (Mascot Delta Score, PTM score and phosphoRS), and we characterized the chromatographic behavior of peptides in the library. We found that HCD identified more peptides and phosphopeptides than did ETD, that phosphopeptides generally eluted later from reversed-phase columns and were easier to identify than unmodified peptides and that current computational tools for proteomics can still be substantially improved. These peptides and spectra will facilitate the development, evaluation and improvement of experimental and computational proteomic strategies, such as separation techniques and the prediction of retention times and fragmentation patterns. |
spellingShingle | Marx, H Lemeer, S Schliep, J Matheron, L Mohammed, S Cox, J Mann, M Heck, A Kuster, B A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics. |
title | A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics. |
title_full | A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics. |
title_fullStr | A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics. |
title_full_unstemmed | A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics. |
title_short | A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics. |
title_sort | large synthetic peptide and phosphopeptide reference library for mass spectrometry based proteomics |
work_keys_str_mv | AT marxh alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT lemeers alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT schliepj alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT matheronl alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT mohammeds alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT coxj alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT mannm alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT hecka alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT kusterb alargesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT marxh largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT lemeers largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT schliepj largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT matheronl largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT mohammeds largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT coxj largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT mannm largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT hecka largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics AT kusterb largesyntheticpeptideandphosphopeptidereferencelibraryformassspectrometrybasedproteomics |