Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients.
The assessment of the TCR repertoire expressed by tumor-specific CD8+ T lymphocytes has been hampered to date by the difficulty of targeting the analysis to lymphocytes directed against a single epitope. In the present study we have used fluorescent A2/Melan-A tetramers in conjunction with anti-CD8...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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| _version_ | 1797067208924332032 |
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| author | Valmori, D Dutoit, V Liénard, D Lejeune, F Speiser, D Rimoldi, D Cerundolo, V Dietrich, P Cerottini, J Romero, P |
| author_facet | Valmori, D Dutoit, V Liénard, D Lejeune, F Speiser, D Rimoldi, D Cerundolo, V Dietrich, P Cerottini, J Romero, P |
| author_sort | Valmori, D |
| collection | OXFORD |
| description | The assessment of the TCR repertoire expressed by tumor-specific CD8+ T lymphocytes has been hampered to date by the difficulty of targeting the analysis to lymphocytes directed against a single epitope. In the present study we have used fluorescent A2/Melan-A tetramers in conjunction with anti-CD8 and anti-TCR beta-chain variable (BV) mAbs to analyze by flow cytometry the BV segment usage by Melan-A-specific CD8+ T cells in tumor-infiltrated lymph nodes (TILN) and tumor-infiltrating lymphocytes (TIL) from A2 melanoma patients. Analysis of TILN populations revealed small proportions of A2/Melan-A tetramer+ cells expressing many different BV together with over-representation of A2/Melan-A tetramer+ cells expressing certain BVs. The BV usage by A2/Melan-A tetramer+ lymphocytes in TIL was more restricted than that in TILN. Moreover, the predominant BV segments were quite distinct in populations derived from different patients. A2/Melan-A tetramer+ cells expressing the dominant BVs found in TILN could also be found in the corresponding peptide-stimulated autologous PBMC, although A2/Melan-A tetramer+ lymphocytes expressing additional BVs were also identified. Together, these results suggest that a large and diverse repertoire of Melan-A-specific T cells using different BV TCR segments is available in A2 melanoma patients. |
| first_indexed | 2024-03-06T21:53:05Z |
| format | Journal article |
| id | oxford-uuid:4bfb8a1b-98a6-409f-a900-ff6ef9649134 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:53:05Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:4bfb8a1b-98a6-409f-a900-ff6ef96491342022-03-26T15:46:47ZTetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4bfb8a1b-98a6-409f-a900-ff6ef9649134EnglishSymplectic Elements at Oxford2000Valmori, DDutoit, VLiénard, DLejeune, FSpeiser, DRimoldi, DCerundolo, VDietrich, PCerottini, JRomero, PThe assessment of the TCR repertoire expressed by tumor-specific CD8+ T lymphocytes has been hampered to date by the difficulty of targeting the analysis to lymphocytes directed against a single epitope. In the present study we have used fluorescent A2/Melan-A tetramers in conjunction with anti-CD8 and anti-TCR beta-chain variable (BV) mAbs to analyze by flow cytometry the BV segment usage by Melan-A-specific CD8+ T cells in tumor-infiltrated lymph nodes (TILN) and tumor-infiltrating lymphocytes (TIL) from A2 melanoma patients. Analysis of TILN populations revealed small proportions of A2/Melan-A tetramer+ cells expressing many different BV together with over-representation of A2/Melan-A tetramer+ cells expressing certain BVs. The BV usage by A2/Melan-A tetramer+ lymphocytes in TIL was more restricted than that in TILN. Moreover, the predominant BV segments were quite distinct in populations derived from different patients. A2/Melan-A tetramer+ cells expressing the dominant BVs found in TILN could also be found in the corresponding peptide-stimulated autologous PBMC, although A2/Melan-A tetramer+ lymphocytes expressing additional BVs were also identified. Together, these results suggest that a large and diverse repertoire of Melan-A-specific T cells using different BV TCR segments is available in A2 melanoma patients. |
| spellingShingle | Valmori, D Dutoit, V Liénard, D Lejeune, F Speiser, D Rimoldi, D Cerundolo, V Dietrich, P Cerottini, J Romero, P Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients. |
| title | Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients. |
| title_full | Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients. |
| title_fullStr | Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients. |
| title_full_unstemmed | Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients. |
| title_short | Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients. |
| title_sort | tetramer guided analysis of tcr beta chain usage reveals a large repertoire of melan a specific cd8 t cells in melanoma patients |
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