Mutagenesis studies on the iron binding ligands of clavaminic acid synthase.

Mutagenesis studies on conserved histidine residues identified as possible metal binding ligands in clavaminic acid synthase isozyme 2 were consistent with His-145 and His-280 acting as iron ligands, in support of crystallographic and previous mutagenesis studies. Mutagenesis of the four cysteines a...

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Chi tiết về thư mục
Những tác giả chính: Doan, L, Hassan, A, Lipscomb, S, Dhanda, A, Zhang, Z, Schofield, C
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: 2000
Miêu tả
Tóm tắt:Mutagenesis studies on conserved histidine residues identified as possible metal binding ligands in clavaminic acid synthase isozyme 2 were consistent with His-145 and His-280 acting as iron ligands, in support of crystallographic and previous mutagenesis studies. Mutagenesis of the four cysteines and a glutamine residue, conserved in both clavaminic acid synthase isozymes 1 and 2, demonstrated that none of these residues is essential for activity.