Mutagenesis studies on the iron binding ligands of clavaminic acid synthase.
Mutagenesis studies on conserved histidine residues identified as possible metal binding ligands in clavaminic acid synthase isozyme 2 were consistent with His-145 and His-280 acting as iron ligands, in support of crystallographic and previous mutagenesis studies. Mutagenesis of the four cysteines a...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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| _version_ | 1797067217308745728 |
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| author | Doan, L Hassan, A Lipscomb, S Dhanda, A Zhang, Z Schofield, C |
| author_facet | Doan, L Hassan, A Lipscomb, S Dhanda, A Zhang, Z Schofield, C |
| author_sort | Doan, L |
| collection | OXFORD |
| description | Mutagenesis studies on conserved histidine residues identified as possible metal binding ligands in clavaminic acid synthase isozyme 2 were consistent with His-145 and His-280 acting as iron ligands, in support of crystallographic and previous mutagenesis studies. Mutagenesis of the four cysteines and a glutamine residue, conserved in both clavaminic acid synthase isozymes 1 and 2, demonstrated that none of these residues is essential for activity. |
| first_indexed | 2024-03-06T21:53:13Z |
| format | Journal article |
| id | oxford-uuid:4c05d85e-8afa-4aae-9bd0-91a355dba5d2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:53:13Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:4c05d85e-8afa-4aae-9bd0-91a355dba5d22022-03-26T15:47:00ZMutagenesis studies on the iron binding ligands of clavaminic acid synthase.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4c05d85e-8afa-4aae-9bd0-91a355dba5d2EnglishSymplectic Elements at Oxford2000Doan, LHassan, ALipscomb, SDhanda, AZhang, ZSchofield, CMutagenesis studies on conserved histidine residues identified as possible metal binding ligands in clavaminic acid synthase isozyme 2 were consistent with His-145 and His-280 acting as iron ligands, in support of crystallographic and previous mutagenesis studies. Mutagenesis of the four cysteines and a glutamine residue, conserved in both clavaminic acid synthase isozymes 1 and 2, demonstrated that none of these residues is essential for activity. |
| spellingShingle | Doan, L Hassan, A Lipscomb, S Dhanda, A Zhang, Z Schofield, C Mutagenesis studies on the iron binding ligands of clavaminic acid synthase. |
| title | Mutagenesis studies on the iron binding ligands of clavaminic acid synthase. |
| title_full | Mutagenesis studies on the iron binding ligands of clavaminic acid synthase. |
| title_fullStr | Mutagenesis studies on the iron binding ligands of clavaminic acid synthase. |
| title_full_unstemmed | Mutagenesis studies on the iron binding ligands of clavaminic acid synthase. |
| title_short | Mutagenesis studies on the iron binding ligands of clavaminic acid synthase. |
| title_sort | mutagenesis studies on the iron binding ligands of clavaminic acid synthase |
| work_keys_str_mv | AT doanl mutagenesisstudiesontheironbindingligandsofclavaminicacidsynthase AT hassana mutagenesisstudiesontheironbindingligandsofclavaminicacidsynthase AT lipscombs mutagenesisstudiesontheironbindingligandsofclavaminicacidsynthase AT dhandaa mutagenesisstudiesontheironbindingligandsofclavaminicacidsynthase AT zhangz mutagenesisstudiesontheironbindingligandsofclavaminicacidsynthase AT schofieldc mutagenesisstudiesontheironbindingligandsofclavaminicacidsynthase |