Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning
Type-H capillary endothelial cells control bone formation during embryogenesis and postnatal growth but few signalling mechanisms underpinning this influence have been characterised. Here, we identify a highly expressed type-H endothelial cell protein, Clec14a, and explore its role in coordinating o...
| Asıl Yazarlar: | , , , , , , , , , , , , , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Nature Research
2024
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| _version_ | 1826314695838007296 |
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| author | Neag, G Lewis, J Turner, JD Manning, JE Dean, I Finlay, M Poologasundarampillai, G Woods, J Sahu, MA Khan, KA Begum, J McGettrick, HM Bellantuono, I Heath, V Jones, SW Buckley, CD Bicknell, R Naylor, AJ |
| author_facet | Neag, G Lewis, J Turner, JD Manning, JE Dean, I Finlay, M Poologasundarampillai, G Woods, J Sahu, MA Khan, KA Begum, J McGettrick, HM Bellantuono, I Heath, V Jones, SW Buckley, CD Bicknell, R Naylor, AJ |
| author_sort | Neag, G |
| collection | OXFORD |
| description | Type-H capillary endothelial cells control bone formation during embryogenesis and postnatal growth but few signalling mechanisms underpinning this influence have been characterised. Here, we identify a highly expressed type-H endothelial cell protein, Clec14a, and explore its role in coordinating osteoblast activity. Expression of Clec14a and its ligand, Mmrn2 are high in murine type-H endothelial cells but absent from osteoblasts. Clec14a−/− mice have premature condensation of the type-H vasculature and expanded distribution of osteoblasts and bone matrix, increased long-bone length and bone density indicative of accelerated skeletal development, and enhanced osteoblast maturation. Antibody-mediated blockade of the Clec14a-Mmrn2 interaction recapitulates the Clec14a−/− phenotype. Endothelial cell expression of Clec14a regulates osteoblast maturation and mineralisation activity during postnatal bone development in mice. This finding underscores the importance of type-H capillary control of osteoblast activity in bone formation and identifies a novel mechanism that mediates this vital cellular crosstalk. |
| first_indexed | 2024-12-09T03:11:33Z |
| format | Journal article |
| id | oxford-uuid:4c241b81-7805-40bd-a01b-e63cc1218d09 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:11:33Z |
| publishDate | 2024 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:4c241b81-7805-40bd-a01b-e63cc1218d092024-10-11T20:08:20ZType-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterningJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4c241b81-7805-40bd-a01b-e63cc1218d09EnglishJisc Publications RouterNature Research2024Neag, GLewis, JTurner, JDManning, JEDean, IFinlay, MPoologasundarampillai, GWoods, JSahu, MAKhan, KABegum, JMcGettrick, HMBellantuono, IHeath, VJones, SWBuckley, CDBicknell, RNaylor, AJType-H capillary endothelial cells control bone formation during embryogenesis and postnatal growth but few signalling mechanisms underpinning this influence have been characterised. Here, we identify a highly expressed type-H endothelial cell protein, Clec14a, and explore its role in coordinating osteoblast activity. Expression of Clec14a and its ligand, Mmrn2 are high in murine type-H endothelial cells but absent from osteoblasts. Clec14a−/− mice have premature condensation of the type-H vasculature and expanded distribution of osteoblasts and bone matrix, increased long-bone length and bone density indicative of accelerated skeletal development, and enhanced osteoblast maturation. Antibody-mediated blockade of the Clec14a-Mmrn2 interaction recapitulates the Clec14a−/− phenotype. Endothelial cell expression of Clec14a regulates osteoblast maturation and mineralisation activity during postnatal bone development in mice. This finding underscores the importance of type-H capillary control of osteoblast activity in bone formation and identifies a novel mechanism that mediates this vital cellular crosstalk. |
| spellingShingle | Neag, G Lewis, J Turner, JD Manning, JE Dean, I Finlay, M Poologasundarampillai, G Woods, J Sahu, MA Khan, KA Begum, J McGettrick, HM Bellantuono, I Heath, V Jones, SW Buckley, CD Bicknell, R Naylor, AJ Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning |
| title | Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning |
| title_full | Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning |
| title_fullStr | Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning |
| title_full_unstemmed | Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning |
| title_short | Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning |
| title_sort | type h endothelial cell protein clec14a orchestrates osteoblast activity during trabecular bone formation and patterning |
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