Signature-tagged mutagenesis: barcoding mutants for genome-wide screens.
DNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Sal...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2006
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| _version_ | 1826271242686038016 |
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| author | Mazurkiewicz, P Tang, C Boone, C Holden, D |
| author_facet | Mazurkiewicz, P Tang, C Boone, C Holden, D |
| author_sort | Mazurkiewicz, P |
| collection | OXFORD |
| description | DNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Salmonella; they have yielded a wealth of information on a broad range of biological processes--mainly in bacteria, but also in yeast and other fungi, viruses, parasites and, most recently, in mammalian cells. By combining whole-genome microarrays and comprehensive ordered libraries of mutants, high-throughput functional screens can now be achieved on a genomic scale. |
| first_indexed | 2024-03-06T21:53:35Z |
| format | Journal article |
| id | oxford-uuid:4c246f71-9331-4ebe-942c-c5f6394c8ad6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:53:35Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:4c246f71-9331-4ebe-942c-c5f6394c8ad62022-03-26T15:47:43ZSignature-tagged mutagenesis: barcoding mutants for genome-wide screens.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4c246f71-9331-4ebe-942c-c5f6394c8ad6EnglishSymplectic Elements at Oxford2006Mazurkiewicz, PTang, CBoone, CHolden, DDNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Salmonella; they have yielded a wealth of information on a broad range of biological processes--mainly in bacteria, but also in yeast and other fungi, viruses, parasites and, most recently, in mammalian cells. By combining whole-genome microarrays and comprehensive ordered libraries of mutants, high-throughput functional screens can now be achieved on a genomic scale. |
| spellingShingle | Mazurkiewicz, P Tang, C Boone, C Holden, D Signature-tagged mutagenesis: barcoding mutants for genome-wide screens. |
| title | Signature-tagged mutagenesis: barcoding mutants for genome-wide screens. |
| title_full | Signature-tagged mutagenesis: barcoding mutants for genome-wide screens. |
| title_fullStr | Signature-tagged mutagenesis: barcoding mutants for genome-wide screens. |
| title_full_unstemmed | Signature-tagged mutagenesis: barcoding mutants for genome-wide screens. |
| title_short | Signature-tagged mutagenesis: barcoding mutants for genome-wide screens. |
| title_sort | signature tagged mutagenesis barcoding mutants for genome wide screens |
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