Multinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors
<p><strong>Purpose:</strong> Aging is associated with changes in muscle energy metabolism. Proton (<sup>1</sup>H) and phosphorous (<sup>31</sup>P) magnetic resonance spectroscopy (MRS) has been successfully applied for non-invasive investigation of...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Frontiers Media
2020
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| _version_ | 1826271244302942208 |
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| author | Krumpolec, P Klepochová, R Just, I Tušek Jelenc, M Frollo, I Ukropec, J Ukropcová, B Trattnig, S Krššák, M Valkovic, L |
| author_facet | Krumpolec, P Klepochová, R Just, I Tušek Jelenc, M Frollo, I Ukropec, J Ukropcová, B Trattnig, S Krššák, M Valkovic, L |
| author_sort | Krumpolec, P |
| collection | OXFORD |
| description | <p><strong>Purpose:</strong> Aging is associated with changes in muscle energy metabolism. Proton (<sup>1</sup>H) and phosphorous (<sup>31</sup>P) magnetic resonance spectroscopy (MRS) has been successfully applied for non-invasive investigation of skeletal muscle metabolism. The aim of this study was to detect differences in adenosine triphosphate (ATP) production in the aging muscle by <sup>31</sup>P-MRS and to identify potential changes associated with buffer capacity of muscle carnosine by <sup>1</sup>H-MRS.</p>
<p><strong>Methods:</strong> Fifteen young and nineteen elderly volunteers were examined. <sup>1</sup>H and <sup>31</sup>P-MRS spectra were acquired at high field (7T). The investigation included carnosine quantification using <sup>1</sup>H-MRS and resting and dynamic <sup>31</sup>P-MRS, both including saturation transfer measurements of phosphocreatine (PCr), and inorganic phosphate (Pi)-to-ATP metabolic fluxes.</p>
<p><strong>Results:</strong> Elderly volunteers had higher time constant of PCr recovery (τ<sub><em>PCr</em></sub>) in comparison to the young volunteers. Exercise was connected with significant decrease in PCr-to-ATP flux in both groups. Moreover, PCr-to-ATP flux was significantly higher in young compared to elderly both at rest and during exercise. Similarly, an increment of Pi-to-ATP flux with exercise was found in both groups but the intergroup difference was only observed during exercise. Elderly had lower muscle carnosine concentration and lower postexercise pH. A strong increase in phosphomonoester (PME) concentration was observed with exercise in elderly, and a faster Pi:PCr kinetics was found in young volunteers compared to elderly during the recovery period.</p>
<p><strong>Conclusion:</strong> Observations of a massive increment of PME concentration together with high Pi-to-ATP flux during exercise in seniors refer to decreased ability of the muscle to meet the metabolic requirements of exercise and thus a limited ability of seniors to effectively support the exercise load.</p> |
| first_indexed | 2024-03-06T21:53:36Z |
| format | Journal article |
| id | oxford-uuid:4c263003-0499-4ef7-85ff-57684ed8a648 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:53:36Z |
| publishDate | 2020 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | oxford-uuid:4c263003-0499-4ef7-85ff-57684ed8a6482022-03-26T15:47:50ZMultinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniorsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4c263003-0499-4ef7-85ff-57684ed8a648EnglishSymplectic ElementsFrontiers Media2020Krumpolec, PKlepochová, RJust, ITušek Jelenc, MFrollo, IUkropec, JUkropcová, BTrattnig, SKrššák, MValkovic, L<p><strong>Purpose:</strong> Aging is associated with changes in muscle energy metabolism. Proton (<sup>1</sup>H) and phosphorous (<sup>31</sup>P) magnetic resonance spectroscopy (MRS) has been successfully applied for non-invasive investigation of skeletal muscle metabolism. The aim of this study was to detect differences in adenosine triphosphate (ATP) production in the aging muscle by <sup>31</sup>P-MRS and to identify potential changes associated with buffer capacity of muscle carnosine by <sup>1</sup>H-MRS.</p> <p><strong>Methods:</strong> Fifteen young and nineteen elderly volunteers were examined. <sup>1</sup>H and <sup>31</sup>P-MRS spectra were acquired at high field (7T). The investigation included carnosine quantification using <sup>1</sup>H-MRS and resting and dynamic <sup>31</sup>P-MRS, both including saturation transfer measurements of phosphocreatine (PCr), and inorganic phosphate (Pi)-to-ATP metabolic fluxes.</p> <p><strong>Results:</strong> Elderly volunteers had higher time constant of PCr recovery (τ<sub><em>PCr</em></sub>) in comparison to the young volunteers. Exercise was connected with significant decrease in PCr-to-ATP flux in both groups. Moreover, PCr-to-ATP flux was significantly higher in young compared to elderly both at rest and during exercise. Similarly, an increment of Pi-to-ATP flux with exercise was found in both groups but the intergroup difference was only observed during exercise. Elderly had lower muscle carnosine concentration and lower postexercise pH. A strong increase in phosphomonoester (PME) concentration was observed with exercise in elderly, and a faster Pi:PCr kinetics was found in young volunteers compared to elderly during the recovery period.</p> <p><strong>Conclusion:</strong> Observations of a massive increment of PME concentration together with high Pi-to-ATP flux during exercise in seniors refer to decreased ability of the muscle to meet the metabolic requirements of exercise and thus a limited ability of seniors to effectively support the exercise load.</p> |
| spellingShingle | Krumpolec, P Klepochová, R Just, I Tušek Jelenc, M Frollo, I Ukropec, J Ukropcová, B Trattnig, S Krššák, M Valkovic, L Multinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors |
| title | Multinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors |
| title_full | Multinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors |
| title_fullStr | Multinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors |
| title_full_unstemmed | Multinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors |
| title_short | Multinuclear MRS at 7T uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors |
| title_sort | multinuclear mrs at 7t uncovers exercise driven differences in skeletal muscle energy metabolism between young and seniors |
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