DNA structural changes induced by intermolecular triple helix formation
<p>DNase I footprints of intermolecular DNA triplexes are often accompanied by enhanced cleavage at the 3′-end of the target site at the triplex–duplex junction. We have systematically studied the sequence dependence of this effect by examining oligonucleotide binding to si...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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American Chemical Society
2020
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| _version_ | 1797067321092603904 |
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| author | Sayoh, I Rusling, DA Brown, T Fox, KR |
| author_facet | Sayoh, I Rusling, DA Brown, T Fox, KR |
| author_sort | Sayoh, I |
| collection | OXFORD |
| description | <p>DNase I footprints of intermolecular DNA triplexes are often accompanied by enhanced cleavage at the 3′-end of the target site at the triplex–duplex junction. We have systematically studied the sequence dependence of this effect by examining oligonucleotide binding to sites flanked by each base in turn. For complexes with a terminal T.AT triplet, the greatest enhancement is seen with ApC, followed by ApG and ApT, with the weakest enhancement at ApA. Similar DNase I enhancements were observed for a triplex with a terminal C<sup>+</sup>.GC triplet, though with little difference between the different GpN sites. Enhanced reactivity to diethylpyrocarbonate was observed at As that flank the triplex–duplex junction at <u>A</u><strong>A</strong>A or <u>A</u><strong>A</strong>C but not <u>A</u><strong>A</strong>G or <u>A</u><strong>A</strong>T. Fluorescence melting experiments demonstrated that the flanking base affected the stability with a 4 °C difference in <em>T</em><sub>m</sub> between a flanking C and G. Sequences that produced the strongest enhancement correlated with those having the lower thermal stability. These results are interpreted in terms of oligonucleotide-induced changes in DNA structure and/or flexibility.</p> |
| first_indexed | 2024-03-06T21:54:36Z |
| format | Journal article |
| id | oxford-uuid:4c7933b4-89c9-4a75-978b-559a2831c2e3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:54:36Z |
| publishDate | 2020 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:4c7933b4-89c9-4a75-978b-559a2831c2e32022-03-26T15:49:40ZDNA structural changes induced by intermolecular triple helix formationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4c7933b4-89c9-4a75-978b-559a2831c2e3EnglishSymplectic ElementsAmerican Chemical Society2020Sayoh, IRusling, DABrown, TFox, KR<p>DNase I footprints of intermolecular DNA triplexes are often accompanied by enhanced cleavage at the 3′-end of the target site at the triplex–duplex junction. We have systematically studied the sequence dependence of this effect by examining oligonucleotide binding to sites flanked by each base in turn. For complexes with a terminal T.AT triplet, the greatest enhancement is seen with ApC, followed by ApG and ApT, with the weakest enhancement at ApA. Similar DNase I enhancements were observed for a triplex with a terminal C<sup>+</sup>.GC triplet, though with little difference between the different GpN sites. Enhanced reactivity to diethylpyrocarbonate was observed at As that flank the triplex–duplex junction at <u>A</u><strong>A</strong>A or <u>A</u><strong>A</strong>C but not <u>A</u><strong>A</strong>G or <u>A</u><strong>A</strong>T. Fluorescence melting experiments demonstrated that the flanking base affected the stability with a 4 °C difference in <em>T</em><sub>m</sub> between a flanking C and G. Sequences that produced the strongest enhancement correlated with those having the lower thermal stability. These results are interpreted in terms of oligonucleotide-induced changes in DNA structure and/or flexibility.</p> |
| spellingShingle | Sayoh, I Rusling, DA Brown, T Fox, KR DNA structural changes induced by intermolecular triple helix formation |
| title | DNA structural changes induced by intermolecular triple helix formation |
| title_full | DNA structural changes induced by intermolecular triple helix formation |
| title_fullStr | DNA structural changes induced by intermolecular triple helix formation |
| title_full_unstemmed | DNA structural changes induced by intermolecular triple helix formation |
| title_short | DNA structural changes induced by intermolecular triple helix formation |
| title_sort | dna structural changes induced by intermolecular triple helix formation |
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