Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.

Two new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino...

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Asıl Yazarlar: Shelbourne, M, Brown, T, El-Sagheer, A
Materyal Türü: Journal article
Dil:English
Baskı/Yayın Bilgisi: 2012
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author Shelbourne, M
Brown, T
El-Sagheer, A
Brown, T
author_facet Shelbourne, M
Brown, T
El-Sagheer, A
Brown, T
author_sort Shelbourne, M
collection OXFORD
description Two new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes.
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spelling oxford-uuid:4cbe6bcb-3aaf-4ff9-b61b-0a3ac5e4a9e52022-03-26T15:51:14ZFast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4cbe6bcb-3aaf-4ff9-b61b-0a3ac5e4a9e5EnglishSymplectic Elements at Oxford2012Shelbourne, MBrown, TEl-Sagheer, ABrown, TTwo new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes.
spellingShingle Shelbourne, M
Brown, T
El-Sagheer, A
Brown, T
Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
title Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
title_full Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
title_fullStr Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
title_full_unstemmed Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
title_short Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
title_sort fast and efficient dna crosslinking and multiple orthogonal labelling by copper free click chemistry
work_keys_str_mv AT shelbournem fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry
AT brownt fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry
AT elsagheera fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry
AT brownt fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry