Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
Two new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1826271355440463872 |
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| author | Shelbourne, M Brown, T El-Sagheer, A Brown, T |
| author_facet | Shelbourne, M Brown, T El-Sagheer, A Brown, T |
| author_sort | Shelbourne, M |
| collection | OXFORD |
| description | Two new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes. |
| first_indexed | 2024-03-06T21:55:22Z |
| format | Journal article |
| id | oxford-uuid:4cbe6bcb-3aaf-4ff9-b61b-0a3ac5e4a9e5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:55:22Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:4cbe6bcb-3aaf-4ff9-b61b-0a3ac5e4a9e52022-03-26T15:51:14ZFast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4cbe6bcb-3aaf-4ff9-b61b-0a3ac5e4a9e5EnglishSymplectic Elements at Oxford2012Shelbourne, MBrown, TEl-Sagheer, ABrown, TTwo new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes. |
| spellingShingle | Shelbourne, M Brown, T El-Sagheer, A Brown, T Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
| title | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
| title_full | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
| title_fullStr | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
| title_full_unstemmed | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
| title_short | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
| title_sort | fast and efficient dna crosslinking and multiple orthogonal labelling by copper free click chemistry |
| work_keys_str_mv | AT shelbournem fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry AT brownt fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry AT elsagheera fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry AT brownt fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry |