Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.
Two new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino...
Asıl Yazarlar: | , , |
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Materyal Türü: | Journal article |
Dil: | English |
Baskı/Yayın Bilgisi: |
2012
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_version_ | 1826271355440463872 |
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author | Shelbourne, M Brown, T El-Sagheer, A Brown, T |
author_facet | Shelbourne, M Brown, T El-Sagheer, A Brown, T |
author_sort | Shelbourne, M |
collection | OXFORD |
description | Two new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes. |
first_indexed | 2024-03-06T21:55:22Z |
format | Journal article |
id | oxford-uuid:4cbe6bcb-3aaf-4ff9-b61b-0a3ac5e4a9e5 |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-06T21:55:22Z |
publishDate | 2012 |
record_format | dspace |
spelling | oxford-uuid:4cbe6bcb-3aaf-4ff9-b61b-0a3ac5e4a9e52022-03-26T15:51:14ZFast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4cbe6bcb-3aaf-4ff9-b61b-0a3ac5e4a9e5EnglishSymplectic Elements at Oxford2012Shelbourne, MBrown, TEl-Sagheer, ABrown, TTwo new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes. |
spellingShingle | Shelbourne, M Brown, T El-Sagheer, A Brown, T Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
title | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
title_full | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
title_fullStr | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
title_full_unstemmed | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
title_short | Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry. |
title_sort | fast and efficient dna crosslinking and multiple orthogonal labelling by copper free click chemistry |
work_keys_str_mv | AT shelbournem fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry AT brownt fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry AT elsagheera fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry AT brownt fastandefficientdnacrosslinkingandmultipleorthogonallabellingbycopperfreeclickchemistry |