Viral particles drive rapid differentiation of memory B cells into secondary plasma cells producing increased levels of antibodies.
Extensive studies have been undertaken to describe naive B cells differentiating into memory B cells at a cellular and molecular level. However, relatively little is known about the fate of memory B cells upon Ag re-encounter. We have previously established a system based on virus-like particles (VL...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Association of Immunologists
2014
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| _version_ | 1826271356435562496 |
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| author | Zabel, F Mohanan, D Bessa, J Link, A Fettelschoss, A Saudan, P Kündig, T Bachmann, M |
| author_facet | Zabel, F Mohanan, D Bessa, J Link, A Fettelschoss, A Saudan, P Kündig, T Bachmann, M |
| author_sort | Zabel, F |
| collection | OXFORD |
| description | Extensive studies have been undertaken to describe naive B cells differentiating into memory B cells at a cellular and molecular level. However, relatively little is known about the fate of memory B cells upon Ag re-encounter. We have previously established a system based on virus-like particles (VLPs), which allows tracking of VLP-specific B cells by flow cytometry as well as histology. Using allotype markers, it is possible to adoptively transfer memory B cells into a naive mouse and track responses of naive and memory B cells in the same mouse under physiological conditions. We have observed that VLP-specific memory B cells quickly differentiated into plasma cells that drove the early onset of a strong humoral IgG response. However, neither IgM(+) nor IgG(+) memory B cells proliferated extensively or entered germinal centers. Remarkably, plasma cells derived from memory B cells preferentially homed to the bone marrow earlier and secreted increased levels of Abs when compared with primary plasma cells derived from naive B cells. Hence, memory B cells have the unique phenotype to differentiate into highly effective secondary plasma cells. |
| first_indexed | 2024-03-06T21:55:23Z |
| format | Journal article |
| id | oxford-uuid:4cbf8cb6-f5a4-48cd-ba3a-e2d3ed263829 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:55:23Z |
| publishDate | 2014 |
| publisher | American Association of Immunologists |
| record_format | dspace |
| spelling | oxford-uuid:4cbf8cb6-f5a4-48cd-ba3a-e2d3ed2638292022-03-26T15:51:14ZViral particles drive rapid differentiation of memory B cells into secondary plasma cells producing increased levels of antibodies.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4cbf8cb6-f5a4-48cd-ba3a-e2d3ed263829EnglishSymplectic Elements at OxfordAmerican Association of Immunologists2014Zabel, FMohanan, DBessa, JLink, AFettelschoss, ASaudan, PKündig, TBachmann, MExtensive studies have been undertaken to describe naive B cells differentiating into memory B cells at a cellular and molecular level. However, relatively little is known about the fate of memory B cells upon Ag re-encounter. We have previously established a system based on virus-like particles (VLPs), which allows tracking of VLP-specific B cells by flow cytometry as well as histology. Using allotype markers, it is possible to adoptively transfer memory B cells into a naive mouse and track responses of naive and memory B cells in the same mouse under physiological conditions. We have observed that VLP-specific memory B cells quickly differentiated into plasma cells that drove the early onset of a strong humoral IgG response. However, neither IgM(+) nor IgG(+) memory B cells proliferated extensively or entered germinal centers. Remarkably, plasma cells derived from memory B cells preferentially homed to the bone marrow earlier and secreted increased levels of Abs when compared with primary plasma cells derived from naive B cells. Hence, memory B cells have the unique phenotype to differentiate into highly effective secondary plasma cells. |
| spellingShingle | Zabel, F Mohanan, D Bessa, J Link, A Fettelschoss, A Saudan, P Kündig, T Bachmann, M Viral particles drive rapid differentiation of memory B cells into secondary plasma cells producing increased levels of antibodies. |
| title | Viral particles drive rapid differentiation of memory B cells into secondary plasma cells producing increased levels of antibodies. |
| title_full | Viral particles drive rapid differentiation of memory B cells into secondary plasma cells producing increased levels of antibodies. |
| title_fullStr | Viral particles drive rapid differentiation of memory B cells into secondary plasma cells producing increased levels of antibodies. |
| title_full_unstemmed | Viral particles drive rapid differentiation of memory B cells into secondary plasma cells producing increased levels of antibodies. |
| title_short | Viral particles drive rapid differentiation of memory B cells into secondary plasma cells producing increased levels of antibodies. |
| title_sort | viral particles drive rapid differentiation of memory b cells into secondary plasma cells producing increased levels of antibodies |
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