2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48.
2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely bloc...
| المؤلفون الرئيسيون: | , , , , , |
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| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
1998
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| _version_ | 1826271398055641088 |
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| author | Brown, M Boles, K Van Der Merwe, P Kumar, V Mathew, P Barclay, A |
| author_facet | Brown, M Boles, K Van Der Merwe, P Kumar, V Mathew, P Barclay, A |
| author_sort | Brown, M |
| collection | OXFORD |
| description | 2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (Kd approximately 16 microM at 37 degreesC) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (Kd approximately 8 microM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation. |
| first_indexed | 2024-03-06T21:56:02Z |
| format | Journal article |
| id | oxford-uuid:4cf7233c-decf-4e3c-ab07-dcc2d2ed65d3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:56:02Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:4cf7233c-decf-4e3c-ab07-dcc2d2ed65d32022-03-26T15:52:33Z2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4cf7233c-decf-4e3c-ab07-dcc2d2ed65d3EnglishSymplectic Elements at Oxford1998Brown, MBoles, KVan Der Merwe, PKumar, VMathew, PBarclay, A2B4 is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. A recombinant protein containing the extracellular region of mouse (m)2B4 attached to avidin-coated fluorescent beads bound to rodent cells, and binding was completely blocked by CD48 monoclonal antibodies (mAbs). Using surface plasmon resonance, we showed that purified soluble mCD48 bound m2B4 with a six- to ninefold higher affinity (Kd approximately 16 microM at 37 degreesC) than its other ligand, CD2. Human CD48 bound human 2B4 with a similar affinity (Kd approximately 8 microM). The finding of an additional ligand for CD48 provides an explanation for distinct functional effects observed on perturbing CD2 and CD48 with mAbs or by genetic manipulation. |
| spellingShingle | Brown, M Boles, K Van Der Merwe, P Kumar, V Mathew, P Barclay, A 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. |
| title | 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. |
| title_full | 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. |
| title_fullStr | 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. |
| title_full_unstemmed | 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. |
| title_short | 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. |
| title_sort | 2b4 the natural killer and t cell immunoglobulin superfamily surface protein is a ligand for cd48 |
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