An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer
Background: Urinary biomarkers are needed to improve the care and reduce the cost of managing bladder cancer. Current biomarkers struggle to identify both high and low-grade cancers due to differing molecular pathways. Changes in microRNA (miR) expression are seen in urothelial carcinogenesis in a p...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2012
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| _version_ | 1797067709980082176 |
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| author | Miah, S Dudziec, E Drayton, R Zlotta, A Morgan, S Rosario, D Hamdy, F Catto, J |
| author_facet | Miah, S Dudziec, E Drayton, R Zlotta, A Morgan, S Rosario, D Hamdy, F Catto, J |
| author_sort | Miah, S |
| collection | OXFORD |
| description | Background: Urinary biomarkers are needed to improve the care and reduce the cost of managing bladder cancer. Current biomarkers struggle to identify both high and low-grade cancers due to differing molecular pathways. Changes in microRNA (miR) expression are seen in urothelial carcinogenesis in a phenotype-specific manner. We hypothesised that urinary miRs reflecting low-and high-grade pathways could detect bladder cancers and overcome differences in genetic events seen within the disease. Methods: We investigated urinary samples (n=121) from patients with bladder cancer (n=68) and age-matched controls (n=53). Fifteen miRs were quantified using real-time PCR. Results: We found that miR is stable within urinary cells despite adverse handling and detected differential expression of 10 miRs from patients with cancer and controls (miRs15a/15b/24-1/27b/100/135b/203/212/328/1224, ANOVA P<0.05). Individually, miR-1224-3p had the best individual performance with specificity, positive and negative predictive values and concordance of 83%, 83%, 75% and 77%, respectively. The combination of miRs-135b/15b/1224-3p detected bladder cancer with a high sensitivity (94.1%), sufficient specificity (51%) and was correct in 86% of patients (concordance). Conclusion: The use of this panel in patients with haematuria would have found 94% of urothelial cell carcinoma, while reducing cystoscopy rates by 26%. However, two invasive cancers (3%) would have been missed. © 2012 Cancer Research UK All rights reserved. |
| first_indexed | 2024-03-06T22:00:11Z |
| format | Journal article |
| id | oxford-uuid:4e4ff690-b610-4f81-b66f-a60f028a3ec3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:00:11Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:4e4ff690-b610-4f81-b66f-a60f028a3ec32022-03-26T16:00:31ZAn evaluation of urinary microRNA reveals a high sensitivity for bladder cancerJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4e4ff690-b610-4f81-b66f-a60f028a3ec3EnglishSymplectic Elements at Oxford2012Miah, SDudziec, EDrayton, RZlotta, AMorgan, SRosario, DHamdy, FCatto, JBackground: Urinary biomarkers are needed to improve the care and reduce the cost of managing bladder cancer. Current biomarkers struggle to identify both high and low-grade cancers due to differing molecular pathways. Changes in microRNA (miR) expression are seen in urothelial carcinogenesis in a phenotype-specific manner. We hypothesised that urinary miRs reflecting low-and high-grade pathways could detect bladder cancers and overcome differences in genetic events seen within the disease. Methods: We investigated urinary samples (n=121) from patients with bladder cancer (n=68) and age-matched controls (n=53). Fifteen miRs were quantified using real-time PCR. Results: We found that miR is stable within urinary cells despite adverse handling and detected differential expression of 10 miRs from patients with cancer and controls (miRs15a/15b/24-1/27b/100/135b/203/212/328/1224, ANOVA P<0.05). Individually, miR-1224-3p had the best individual performance with specificity, positive and negative predictive values and concordance of 83%, 83%, 75% and 77%, respectively. The combination of miRs-135b/15b/1224-3p detected bladder cancer with a high sensitivity (94.1%), sufficient specificity (51%) and was correct in 86% of patients (concordance). Conclusion: The use of this panel in patients with haematuria would have found 94% of urothelial cell carcinoma, while reducing cystoscopy rates by 26%. However, two invasive cancers (3%) would have been missed. © 2012 Cancer Research UK All rights reserved. |
| spellingShingle | Miah, S Dudziec, E Drayton, R Zlotta, A Morgan, S Rosario, D Hamdy, F Catto, J An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer |
| title | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer |
| title_full | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer |
| title_fullStr | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer |
| title_full_unstemmed | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer |
| title_short | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer |
| title_sort | evaluation of urinary microrna reveals a high sensitivity for bladder cancer |
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