Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice
<p>Engineering human tissue with diverse cell types and architectures remains challenging. The cerebral cortex, which has a layered cellular architecture composed of layer-specific neurons organised into vertical columns, delivers higher cognition through intricately wired neural circuits. How...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
Springer Nature
2023
|
| _version_ | 1826312332130648064 |
|---|---|
| author | Jin, Y Mikhailova, E Lei, M Zhou, L Bayley, H Cowley, SA Sun, T Yang, X Zhang, Y Liu, K Catarino da Silva, D Campos Soares, L Bandiera, S Szele, FG Molnár, Z |
| author_facet | Jin, Y Mikhailova, E Lei, M Zhou, L Bayley, H Cowley, SA Sun, T Yang, X Zhang, Y Liu, K Catarino da Silva, D Campos Soares, L Bandiera, S Szele, FG Molnár, Z |
| author_sort | Jin, Y |
| collection | OXFORD |
| description | <p>Engineering human tissue with diverse cell types and architectures remains challenging. The cerebral cortex, which has a layered cellular architecture composed of layer-specific neurons organised into vertical columns, delivers higher cognition through intricately wired neural circuits. However, current tissue engineering approaches cannot produce such structures. Here, we use a droplet printing technique to fabricate tissues comprising simplified cerebral cortical columns. Human induced pluripotent stem cells are differentiated into upper- and deep-layer neural progenitors, which are then printed to form cerebral cortical tissues with a two-layer organization. The tissues show layer-specific biomarker expression and develop a structurally integrated network of processes. Implantation of the printed cortical tissues into ex vivo mouse brain explants results in substantial structural implant-host integration across the tissue boundaries as demonstrated by the projection of processes and the migration of neurons, and leads to the appearance of correlated Ca<sup>2+</sup> oscillations across the interface. The presented approach might be used for the evaluation of drugs and nutrients that promote tissue integration. Importantly, our methodology offers a technical reservoir for future personalized implantation treatments that use 3D tissues derived from a patient’s own induced pluripotent stem cells.</p> |
| first_indexed | 2024-03-07T08:27:28Z |
| format | Journal article |
| id | oxford-uuid:4e6e97f3-9b5f-4c35-a56f-2a8b5f5a2cfd |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:27:28Z |
| publishDate | 2023 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:4e6e97f3-9b5f-4c35-a56f-2a8b5f5a2cfd2024-02-27T07:28:31ZIntegration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain sliceJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4e6e97f3-9b5f-4c35-a56f-2a8b5f5a2cfdEnglishSymplectic ElementsSpringer Nature2023Jin, YMikhailova, ELei, MZhou, LBayley, HCowley, SASun, TYang, XZhang, YLiu, KCatarino da Silva, DCampos Soares, LBandiera, SSzele, FGMolnár, Z<p>Engineering human tissue with diverse cell types and architectures remains challenging. The cerebral cortex, which has a layered cellular architecture composed of layer-specific neurons organised into vertical columns, delivers higher cognition through intricately wired neural circuits. However, current tissue engineering approaches cannot produce such structures. Here, we use a droplet printing technique to fabricate tissues comprising simplified cerebral cortical columns. Human induced pluripotent stem cells are differentiated into upper- and deep-layer neural progenitors, which are then printed to form cerebral cortical tissues with a two-layer organization. The tissues show layer-specific biomarker expression and develop a structurally integrated network of processes. Implantation of the printed cortical tissues into ex vivo mouse brain explants results in substantial structural implant-host integration across the tissue boundaries as demonstrated by the projection of processes and the migration of neurons, and leads to the appearance of correlated Ca<sup>2+</sup> oscillations across the interface. The presented approach might be used for the evaluation of drugs and nutrients that promote tissue integration. Importantly, our methodology offers a technical reservoir for future personalized implantation treatments that use 3D tissues derived from a patient’s own induced pluripotent stem cells.</p> |
| spellingShingle | Jin, Y Mikhailova, E Lei, M Zhou, L Bayley, H Cowley, SA Sun, T Yang, X Zhang, Y Liu, K Catarino da Silva, D Campos Soares, L Bandiera, S Szele, FG Molnár, Z Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice |
| title | Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice |
| title_full | Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice |
| title_fullStr | Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice |
| title_full_unstemmed | Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice |
| title_short | Integration of 3D-printed cerebral cortical tissue into an ex vivo lesioned brain slice |
| title_sort | integration of 3d printed cerebral cortical tissue into an ex vivo lesioned brain slice |
| work_keys_str_mv | AT jiny integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT mikhailovae integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT leim integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT zhoul integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT bayleyh integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT cowleysa integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT sunt integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT yangx integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT zhangy integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT liuk integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT catarinodasilvad integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT campossoaresl integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT bandieras integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT szelefg integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice AT molnarz integrationof3dprintedcerebralcorticaltissueintoanexvivolesionedbrainslice |