Interferometric scattering microscopy for the study of molecular motors
Our understanding of molecular motor function has been greatly improved by the development of imaging modalities, which enable real-time observation of their motion at the single-molecule level. Here, we describe the use of a new method, interferometric scattering microscopy, for the investigation o...
| 主要な著者: | , , , , , , , |
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| フォーマット: | Book section |
| 言語: | English |
| 出版事項: |
Elsevier
2016
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| _version_ | 1826271691090690048 |
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| author | Andrecka, J Takagi, Y Mickolajczyk, K Lippert, L Sellers, J Hancock, W Goldman, Y Kukura, P |
| author_facet | Andrecka, J Takagi, Y Mickolajczyk, K Lippert, L Sellers, J Hancock, W Goldman, Y Kukura, P |
| author_sort | Andrecka, J |
| collection | OXFORD |
| description | Our understanding of molecular motor function has been greatly improved by the development of imaging modalities, which enable real-time observation of their motion at the single-molecule level. Here, we describe the use of a new method, interferometric scattering microscopy, for the investigation of motor protein dynamics by attaching and tracking the motion of metallic nanoparticle labels as small as 20 nm diameter. Using myosin-5, kinesin-1, and dynein as examples, we describe the basic assays, labeling strategies, and principles of data analysis. Our approach is relevant not only for motor protein dynamics but also provides a general tool for single-particle tracking with high spatiotemporal precision, which overcomes the limitations of single-molecule fluorescence methods. |
| first_indexed | 2024-03-06T22:00:39Z |
| format | Book section |
| id | oxford-uuid:4e7b2e2e-ff46-4c41-a10c-f482511c3cf1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:00:39Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:4e7b2e2e-ff46-4c41-a10c-f482511c3cf12022-03-26T16:01:25ZInterferometric scattering microscopy for the study of molecular motorsBook sectionhttp://purl.org/coar/resource_type/c_3248uuid:4e7b2e2e-ff46-4c41-a10c-f482511c3cf1EnglishSymplectic Elements at OxfordElsevier2016Andrecka, JTakagi, YMickolajczyk, KLippert, LSellers, JHancock, WGoldman, YKukura, POur understanding of molecular motor function has been greatly improved by the development of imaging modalities, which enable real-time observation of their motion at the single-molecule level. Here, we describe the use of a new method, interferometric scattering microscopy, for the investigation of motor protein dynamics by attaching and tracking the motion of metallic nanoparticle labels as small as 20 nm diameter. Using myosin-5, kinesin-1, and dynein as examples, we describe the basic assays, labeling strategies, and principles of data analysis. Our approach is relevant not only for motor protein dynamics but also provides a general tool for single-particle tracking with high spatiotemporal precision, which overcomes the limitations of single-molecule fluorescence methods. |
| spellingShingle | Andrecka, J Takagi, Y Mickolajczyk, K Lippert, L Sellers, J Hancock, W Goldman, Y Kukura, P Interferometric scattering microscopy for the study of molecular motors |
| title | Interferometric scattering microscopy for the study of molecular motors |
| title_full | Interferometric scattering microscopy for the study of molecular motors |
| title_fullStr | Interferometric scattering microscopy for the study of molecular motors |
| title_full_unstemmed | Interferometric scattering microscopy for the study of molecular motors |
| title_short | Interferometric scattering microscopy for the study of molecular motors |
| title_sort | interferometric scattering microscopy for the study of molecular motors |
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