Adoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice.
Immune depression associated with prepubescent malnutrition underlies a staggering burden of infection-related morbidity. This investigation centered on dendritic cells as potentially decisive in this phenomenon. C57BL/6J mice, initially 19 days old, had free access for 14 days to a complete diet or...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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2008
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| _version_ | 1797067944039022592 |
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| author | Hillyer, L Whitley, C Olver, A Webster, M Steevels, T Woodward, B |
| author_facet | Hillyer, L Whitley, C Olver, A Webster, M Steevels, T Woodward, B |
| author_sort | Hillyer, L |
| collection | OXFORD |
| description | Immune depression associated with prepubescent malnutrition underlies a staggering burden of infection-related morbidity. This investigation centered on dendritic cells as potentially decisive in this phenomenon. C57BL/6J mice, initially 19 days old, had free access for 14 days to a complete diet or to a low-protein formulation that induced wasting deficits of protein and energy. Mice were sensitized by i.p. injection of sheep red blood cells on day 9, at which time one-half of the animals in each dietary group received a simultaneous injection of 10(6) syngeneic dendritic cells (JAWS II). All mice were challenged with the immunizing antigen in the right hind footpad on day 13, and the 24-hour delayed hypersensitivity response was assessed as percentage increase in footpad thickness. The low-protein diet reduced the inflammatory immune response, but JAWS cells, which exhibited immature phenotypic and functional characteristics, increased the response of both the malnourished group and the controls. By contrast, i.p. injection of 10(6) syngeneic T cells did not influence the inflammatory immune response of mice subjected to the low-protein protocol. Antigen-presenting cell numbers limited primary inflammatory cell-mediated competence in this model of wasting malnutrition, an outcome that challenges the prevailing multifactorial model of malnutrition-associated immune depression. Thus, a new dendritic cell-centered perspective emerges regarding the cellular mechanism underlying immune depression in acute pediatric protein and energy deficit. |
| first_indexed | 2024-03-06T22:03:39Z |
| format | Journal article |
| id | oxford-uuid:4f6cd95c-c301-490e-ac1f-b904b1a568f0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T22:03:39Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:4f6cd95c-c301-490e-ac1f-b904b1a568f02022-03-26T16:07:10ZAdoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4f6cd95c-c301-490e-ac1f-b904b1a568f0EnglishSymplectic Elements at Oxford2008Hillyer, LWhitley, COlver, AWebster, MSteevels, TWoodward, BImmune depression associated with prepubescent malnutrition underlies a staggering burden of infection-related morbidity. This investigation centered on dendritic cells as potentially decisive in this phenomenon. C57BL/6J mice, initially 19 days old, had free access for 14 days to a complete diet or to a low-protein formulation that induced wasting deficits of protein and energy. Mice were sensitized by i.p. injection of sheep red blood cells on day 9, at which time one-half of the animals in each dietary group received a simultaneous injection of 10(6) syngeneic dendritic cells (JAWS II). All mice were challenged with the immunizing antigen in the right hind footpad on day 13, and the 24-hour delayed hypersensitivity response was assessed as percentage increase in footpad thickness. The low-protein diet reduced the inflammatory immune response, but JAWS cells, which exhibited immature phenotypic and functional characteristics, increased the response of both the malnourished group and the controls. By contrast, i.p. injection of 10(6) syngeneic T cells did not influence the inflammatory immune response of mice subjected to the low-protein protocol. Antigen-presenting cell numbers limited primary inflammatory cell-mediated competence in this model of wasting malnutrition, an outcome that challenges the prevailing multifactorial model of malnutrition-associated immune depression. Thus, a new dendritic cell-centered perspective emerges regarding the cellular mechanism underlying immune depression in acute pediatric protein and energy deficit. |
| spellingShingle | Hillyer, L Whitley, C Olver, A Webster, M Steevels, T Woodward, B Adoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice. |
| title | Adoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice. |
| title_full | Adoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice. |
| title_fullStr | Adoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice. |
| title_full_unstemmed | Adoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice. |
| title_short | Adoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice. |
| title_sort | adoptively transferred dendritic cells restore primary cell mediated inflammatory competence to acutely malnourished weanling mice |
| work_keys_str_mv | AT hillyerl adoptivelytransferreddendriticcellsrestoreprimarycellmediatedinflammatorycompetencetoacutelymalnourishedweanlingmice AT whitleyc adoptivelytransferreddendriticcellsrestoreprimarycellmediatedinflammatorycompetencetoacutelymalnourishedweanlingmice AT olvera adoptivelytransferreddendriticcellsrestoreprimarycellmediatedinflammatorycompetencetoacutelymalnourishedweanlingmice AT websterm adoptivelytransferreddendriticcellsrestoreprimarycellmediatedinflammatorycompetencetoacutelymalnourishedweanlingmice AT steevelst adoptivelytransferreddendriticcellsrestoreprimarycellmediatedinflammatorycompetencetoacutelymalnourishedweanlingmice AT woodwardb adoptivelytransferreddendriticcellsrestoreprimarycellmediatedinflammatorycompetencetoacutelymalnourishedweanlingmice |